ε Tubulin CRISPR/Cas9 KO Plasmid (h): sc-405619
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ε Tubulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ε Tubulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ε Tubulin Antibody (5F3B7): sc-517236
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ε Tubulin CRISPR/Cas9 KO Plasmid (h) | sc-405619 | 20 µg | $397.00 |
Overview
TUBE1 encodes ε-tubulin, a highly conserved member of the tubulin family implicated in centriole biology and microtubule-based organization. ε-tubulin has been associated with centriole duplication and centrosome integrity, processes that coordinate mitotic spindle assembly, chromosome segregation, and cell-cycle progression. Disruption of centrosome homeostasis is linked to genomic instability and altered proliferative signaling, connecting tubulin-network perturbations to cancer-relevant phenotypes. As a centriole-associated tubulin, ε-tubulin is also of interest for studying cell division defects and microtubule-dependent trafficking in human cells.
ε Tubulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TUBE1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TUBE1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TUBE1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ε Tubulin protein expression.
This CRISPR knockout system enables efficient generation of TUBE1-deficient cell models for investigation of ε Tubulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TUBE1 exon(s) critical for ε Tubulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TUBE1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ε Tubulin CRISPR/Cas9 KO Plasmid (h) and ε Tubulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TUBE1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ε Tubulin HDR Plasmid (h) and ε Tubulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TUBE1 homology arms to support homology-directed repair at defined TUBE1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.