α-parvin CRISPR/Cas9 KO Plasmid (h): sc-405769
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α-parvin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α-parvin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-parvin CRISPR/Cas9 KO Plasmid (h) | sc-405769 | 20 µg | $397.00 |
Overview
PARVA encodes α-parvin, an actin-binding focal adhesion adaptor that associates with integrin-linked kinase (ILK) and PINCH to form the IPP complex, linking integrins to the actin cytoskeleton. Through interactions with paxillin and other adhesion components, α-parvin contributes to focal adhesion assembly, cytoskeletal remodeling, and mechanotransduction during cell spreading and migration. PARVA-dependent signaling interfaces with integrin/FAK pathways and small GTPase-regulated actin dynamics, supporting adhesion-dependent survival and cellular polarity. Dysregulation of these processes is relevant to studies of tumor cell invasion, fibrosis-related tissue remodeling, and cardiovascular and smooth muscle biology where altered adhesion and contractile signaling are implicated.
α-parvin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PARVA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PARVA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PARVA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α-parvin protein expression.
This CRISPR knockout system enables efficient generation of PARVA-deficient cell models for investigation of α-parvin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PARVA exon(s) critical for α-parvin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PARVA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α-parvin CRISPR/Cas9 KO Plasmid (h) and α-parvin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PARVA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α-parvin HDR Plasmid (h) and α-parvin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PARVA homology arms to support homology-directed repair at defined PARVA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.