TRM61 Antibody (C145I165): sc-81062

TRM61 Antibody (C145I165) is a mouse monoclonal IgG1 antibody that detects TRM61 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-TRM61 antibody (C145I165) is available as the non-conjugated format. TRM61, also known as tRNA m(1)A58 methyltransferase subunit TRM61 or GCD14, is a vital component of the tRNA m(1)A58 methyltransferase complex, which consists of two subunits, TRM61 and TRM6, that function as heterotetramers. This enzyme is responsible for the methylation of adenosine at position 58 in tRNA, resulting in the formation of 1-methyladenosine, a crucial modification that stabilizes the initiator methionine tRNA (tRNAiMet). The presence of 1-methyladenosine is essential for the proper folding and function of tRNAiMet, which plays a direct role in the initiation of protein synthesis. Therefore, TRM61 is critical for maintaining the integrity of tRNA structure, and any mutations in the TRM61 gene that alter the substrate-binding pocket of the methyltransferase can lead to tRNAiMet instability, potentially disrupting protein synthesis and cellular function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TRM61 Antibody (C145I165) References:

1. GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in the maturation of initiator methionyl-tRNA in Saccharomyces cerevisiae.  |  Calvo, O., et al. 1999. Mol Cell Biol. 19: 4167-81. PMID: 10330157
2. The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae.  |  Anderson, J., et al. 2000. Proc Natl Acad Sci U S A. 97: 5173-8. PMID: 10779558
3. In silico analysis of the tRNA:m1A58 methyltransferase family: homology-based fold prediction and identification of new members from Eubacteria and Archaea.  |  Bujnicki, JM. 2001. FEBS Lett. 507: 123-7. PMID: 11684083
4. Nuclear surveillance and degradation of hypomodified initiator tRNAMet in S. cerevisiae.  |  Kadaba, S., et al. 2004. Genes Dev. 18: 1227-40. PMID: 15145828
5. Role of a 300-kilodalton nuclear complex in the maturation of Trypanosoma brucei initiator methionyl-tRNA.  |  Arhin, GK., et al. 2004. Eukaryot Cell. 3: 893-9. PMID: 15302822
6. Detection and discovery of RNA modifications using microarrays.  |  Hiley, SL., et al. 2005. Nucleic Acids Res. 33: e2. PMID: 15640439
7. The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans.  |  Ozanick, S., et al. 2005. RNA. 11: 1281-90. PMID: 16043508
8. Conserved amino acids in each subunit of the heteroligomeric tRNA m1A58 Mtase from Saccharomyces cerevisiae contribute to tRNA binding.  |  Ozanick, SG., et al. 2007. Nucleic Acids Res. 35: 6808-19. PMID: 17932071
9. Two-subunit enzymes involved in eukaryotic post-transcriptional tRNA modification.  |  Guy, MP. and Phizicky, EM. 2014. RNA Biol. 11: 1608-18. PMID: 25625329
10. m1A Post-Transcriptional Modification in tRNAs.  |  Oerum, S., et al. 2017. Biomolecules. 7: PMID: 28230814
11. The Arabidopsis TRM61/TRM6 complex is a bona fide tRNA N1-methyladenosine methyltransferase.  |  Tang, J., et al. 2020. J Exp Bot. 71: 3024-3036. PMID: 32095811
12. The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA.  |  Anderson, J., et al. 1998. Genes Dev. 12: 3650-62. PMID: 9851972

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRM61 Antibody (C145I165) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TRM61 Antibody (C145I165) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRM61 Antibody (C145I165) Specifications

Alternate Product Name: TRM61

Product Clone Name: C145I165

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: TRM61

Epitope Species: human

Product Reactivity/Detection: TRM61

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TRM61 Antibody (C145I165): N/A