Anticorpo SREBP-2 (C-6) raccomandato per il rilevamento di SREBP-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo SREBP-2 (C-6): sc-271615

Anticorpo SREBP-2 (C-6) valutazione 5.0 di 5 di 2.
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Schede tecniche

    • SREBP-2 Antibody C-6 è un monoclonale di topo IgG2a κ, citato in 6 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 812-975 di SREBP-2 di origine human
    • raccomandato per il rilevamento di SREBP-2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • Vedere SREBP-2 (1C6): sc-13552 for SREBP-2 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for SREBP-2 Antibody (C-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with SREBP-2 Antibody (C-6) (see ordering information below).
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Why do your WBlott samples (Data sh.) as well as the above citations (Liu et al) show a 55/68 kDa mark if the antibody was performed to the C-terminal part of the protein ( not present in the mature form of SREBP) ??? Isn´t that inespecific??

Richiesta da: tesl
Thank you for your question. There are known cleavage and degradation products for SERBP-2 that run as low as 55kDa, so it's possible to see signal for this protein ranging from that size and up to mature form around 68-70kDa and pre-cursor around 125kDa. It is very dependent on your samples and the status of the protein in your samples.
Risposta da: Tech Service
Data di pubblicazione: 2020-01-16

I am using SREBP-2 (C-6): sc-271615 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Richiesta da: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 5 di 5 di da Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Data di pubblicazione: 2015-03-26
Rated 5 di 5 di da Great antibody for Western Blot Great antibody for Western Blot, nice signal on LNCaP whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-02-02
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