Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Smad8 Double Nickase Plasmid (h): sc-402119-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Smad8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Smad8 Double Nickase Plasmid (h) and Smad8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SMAD9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Smad8 Antibody (3E5): sc-293413
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smad8 Double Nickase Plasmid (h) | sc-402119-NIC | 20 µg | $410.00 | |||
Smad8 Double Nickase Plasmid (h2) | sc-402119-NIC-2 | 20 µg | $410.00 |
SMAD9 encodes Smad8, a receptor-regulated SMAD that transduces bone morphogenetic protein (BMP) signals from activated type I receptors to the nucleus. Following C-terminal phosphorylation, Smad8 forms complexes with SMAD4 and coordinates transcriptional programs controlling osteogenic and chondrogenic differentiation, extracellular matrix remodeling, and developmental patterning. SMAD9-dependent BMP/SMAD signaling intersects with MAPK and other context-specific pathways to tune signal amplitude and duration. Dysregulation of this axis has been associated with altered skeletal homeostasis and vascular remodeling phenotypes, supporting mechanistic studies of SMAD9 in lineage commitment and tissue remodeling models.
Smad8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SMAD9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SMAD9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SMAD9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SMAD9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.