Smad8 Antibody (3E5): sc-293413
- Smad8 Antibody (3E5) is a mouse monoclonal IgG2a κ Smad8 antibody, cited in 1 publications, provided at 100 µg/ml
- raised against a GST fusion protein corresponding to amino acids 146-260 representing full length Smad8 of human origin
- recommended for detection of Smad8 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Smad8 Antibody (3E5). This work is in progress.
Smad8 Antibody (3E5) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Smad8 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-Smad8 antibody (3E5) is available as a non-conjugated isotype antibody. Smad8, also known as Smad9 or MADH6, plays a crucial role in the bone morphogenetic protein (BMP) signaling pathway, which is essential for various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis. Located primarily in the cytoplasm and nucleus, Smad8 transduces signals from BMP receptors to the nucleus, where Smad8 regulates gene expression. This localization allows Smad8 to act as a transcriptional regulator, influencing the expression of target genes involved in developmental processes and cellular responses to environmental cues. Proper functioning of Smad8 is critical, as dysregulation of BMP signaling has been implicated in various diseases, including cancer and fibrotic disorders. Using Smad8 (3E5) antibody in research can provide insights into the mechanisms of BMP signaling and its implications in health and disease.
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Smad8 Antibody (3E5) References:
- Mouse smad8 phosphorylation downstream of BMP receptors ALK-2, ALK-3, and ALK-6 induces its association with Smad4 and transcriptional activity. | Kawai, S., et al. 2000. Biochem Biophys Res Commun. 271: 682-7. PMID: 10814522
- Elucidation of epigenetic inactivation of SMAD8 in cancer using targeted expressed gene display. | Cheng, KH., et al. 2004. Cancer Res. 64: 1639-46. PMID: 14996722
- Smad1 and Smad8 function similarly in mammalian central nervous system development. | Hester, M., et al. 2005. Mol Cell Biol. 25: 4683-92. PMID: 15899870
- Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells. | Hoffmann, A., et al. 2006. J Clin Invest. 116: 940-52. PMID: 16585960
- A new nonsense mutation of SMAD8 associated with pulmonary arterial hypertension. | Shintani, M., et al. 2009. J Med Genet. 46: 331-7. PMID: 19211612
- Smad8 is expressed in the anterior necrotic zone: evidence for a role of bone morphogenetic proteins/SMAD signaling in the activation of a molecular cascade that culminates in cell death. | Abarca-Buis, RF., et al. 2011. Dev Growth Differ. 53: 780-92. PMID: 21711459
- Smad8/BMP2-engineered mesenchymal stem cells induce accelerated recovery of the biomechanical properties of the Achilles tendon. | Pelled, G., et al. 2012. J Orthop Res. 30: 1932-9. PMID: 22696396
- Smad8/9 Is Regulated Through the BMP Pathway. | Katakawa, Y., et al. 2016. J Cell Biochem. 117: 1788-96. PMID: 26748560
- Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3). | Segovia-Trinidad, CL., et al. 2021. Protein Expr Purif. 184: 105878. PMID: 33812004
- Smad8 Is Increased in Duchenne Muscular Dystrophy and Suppresses miR-1, miR-133a, and miR-133b. | Lopez, MA., et al. 2022. Int J Mol Sci. 23: PMID: 35886863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smad8 Antibody (3E5) | sc-293413 | 100 µg/ml | $322.00 |