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Rock-2 Antibody (D-2) - Western Blotting - Image 305691
Rock-2 Antibody (D-2): sc-365275. Western blot analysis of Rock-2 expression in HeLa (A), A-673 (B), Y79 (C), MCF7 (D) and Jurkat (E) whole cell lysates.
Rock-2 Antibody (D-2) - Western Blotting - Image 305691
Rock-2 Antibody (D-2): sc-365275. Western blot analysis of Rock-2 expression in HeLa (A), A-673 (B), Y79 (C), MCF7 (D) and Jurkat (E) whole cell lysates.
Rock-2 Antibody (D-2) - Western Blotting - Image 138939
Rock-2 Antibody (D-2): sc-365275. Western blot analysis of Rock-2 expression in Sol8 whole cell lysate.
Rock-2 Antibody (D-2): sc-365275. Western blot analysis of Rock-2 expression in HeLa (A), A-673 (B), Y79 (C), MCF7 (D) and Jurkat (E) whole cell lysates.

Rock-2 Antibody (D-2): sc-365275

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Datasheets
  • Rock-2 Antibody (D-2) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 1353-1379 at the C-terminus of Rock-2 of rat origin
  • recommended for detection of Rock-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and bovine
  • See Rock-2 (D-11): sc-398519 for Rock-2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG2b BP-HRP is the preferred secondary detection reagent for Rock-2 Antibody (D-2) for WB applications. This reagent is now offered in a bundle with Rock-2 Antibody (D-2) (see ordering information below).
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Additional Rock Antibodies including Rock-1 and Rock-2

Rock-2 Antibody (D-2) is a mouse monoclonal IgG2b antibody that detects Rock-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rock-2, also known as Rho-associated coiled-coil containing protein kinase 2, plays a crucial role in regulating various cellular processes, including cell migration, proliferation, and apoptosis, by acting as a downstream effector of the Rho GTPase signaling pathway. Rock-2 (D-2) antibody helps researchers study Rock-2′s ability to modulate the actin cytoskeleton, which is essential for maintaining cell shape and facilitating movement. Rock-2 is involved in the phosphorylation of myosin light chain, a key step in muscle contraction and cellular tension generation. Understanding Rock-2 function is vital, as dysregulation has been implicated in various diseases, including cancer and cardiovascular disorders. Anti-Rock-2 antibody (D-2) provides a reliable tool for researchers aiming to explore Rock-2′s intricate roles in cellular dynamics and potential as a therapeutic target.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Rock-2 Antibody (D-2) References:

  1. Upregulation of Rho-kinase (ROCK-2) expression and enhanced contraction to endothelin-1 in the mesenteric artery from lipopolysaccharide-treated rats.  |  Büyükafşar, K., et al. 2004. Eur J Pharmacol. 498: 211-7. PMID: 15363997
  2. Rho-kinase (ROCK-1 and ROCK-2) upregulation in oleic acid-induced lung injury and its restoration by Y-27632.  |  Köksel, O., et al. 2005. Eur J Pharmacol. 510: 135-42. PMID: 15740734
  3. Nitric oxide does not downregulate Rho-kinase (ROCK-2) expression in rat coronary endothelial cells.  |  Tiftik, RN., et al. 2008. J Cardiovasc Pharmacol. 51: 140-7. PMID: 18287881
  4. Participation of Rho, ROCK-2, and GAP activities during actin microfilament rearrangements in Entamoeba histolytica induced by fibronectin signaling.  |  Ríos, A., et al. 2008. Cell Biol Int. 32: 984-1000. PMID: 18501645
  5. Ouabain-induced apoptosis and Rho kinase: a novel caspase-2 cleavage site and fragment of Rock-2.  |  Ark, M., et al. 2010. Apoptosis. 15: 1494-506. PMID: 20661774
  6. Aldosterone induction of hepatic stellate cell contraction through activation of RhoA/ROCK-2 signaling pathway.  |  Ji, H., et al. 2011. Regul Pept. 169: 13-20. PMID: 21545816
  7. G protein-coupled estrogen receptor1 (GPER1) may mediate Rho-kinase (ROCK-2) up-regulation in coronary endothelial cells.  |  Kurt, AH., et al. 2013. Endocr Regul. 47: 75-84. PMID: 23641788
  8. Testosterone promotes vascular endothelial cell migration via upregulation of ROCK-2/moesin cascade.  |  Liao, W., et al. 2013. Mol Biol Rep. 40: 6729-35. PMID: 24065547
  9. Bioremediation in Fractured Rock: 2. Mobilization of Chloroethene Compounds from the Rock Matrix.  |  Shapiro, AM., et al. 2018. Ground Water. 56: 317-336. PMID: 28873499
  10. RhoA/ROCK-2 Pathway Inhibition and Tight Junction Protein Upregulation by Catalpol Suppresses Lipopolysaccaride-Induced Disruption of Blood-Brain Barrier Permeability.  |  Feng, S., et al. 2018. Molecules. 23: PMID: 30227623

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rock-2 Antibody (D-2)

sc-365275
200 µg/ml
$322.00

Rock-2 Antibody (D-2): m-IgG2b BP-HRP Bundle

sc-548551
200 µg Ab; 10 µg BP
$361.00

Rock-2 (D-2) Neutralizing Peptide

sc-365275 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-365275 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by QZ from Good Western Blot data of RockGood Western Blot data of Rock-2 expression in Sol8 whole cell lysate. -SCBT QC
Date published: 2015-05-01
Rated 3 out of 5 by Anna from No commentNo comment
Date published: 2015-03-23
Rated 5 out of 5 by CJ from Great Western Blot data of RockGreat Western Blot data of Rock-2 expression in HeLa, A-673, Y79, MCF7 and Jurkat whole cell lysates. -SCBT QC
Date published: 2014-03-07
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Rock-2 Antibody (D-2) is rated 4.3 out of 5 by 3.
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