PTTG Antibody (DCS-280): sc-56207

PTTG Antibody (DCS-280) is a mouse monoclonal IgG2a kappa light chain antibody that detects PTTG protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PTTG (DCS-280) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PTTG protein, also known as pituitary tumor-transforming gene, plays a crucial role in cell cycle regulation by acting as an anaphase inhibitor, specifically regulating separin Esp1, which is essential for chromatid separation during mitosis. This function helps ensure proper chromosome segregation, thereby preventing chromosomal instability, a hallmark of many cancers. PTTG protein is predominantly localized in the cytoplasm but is also found in the nucleus, indicating involvement in multiple cellular processes. PTTG protein is overexpressed in certain cancer cell lines, such as Jurkat cells, and is detected in various human tissues, including thymus, testis, and placenta. Unique structural features of PTTG protein, including basic amino-terminal and acidic carboxy-terminal domains, allow function as a transactivator when fused to other DNA-binding domains, further underscoring importance in cellular regulation and potential implications in oncogenesis.

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PTTG Antibody (DCS-280) References:

1. Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1.  |  Uhlmann, F., et al. 1999. Nature. 400: 37-42. PMID: 10403247
2. Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis.  |  Zou, H., et al. 1999. Science. 285: 418-22. PMID: 10411507
3. PTTG overexpression is correlated with angiogenesis in human pituitary adenomas.  |  Minematsu, T., et al. 2006. Endocr Pathol. 17: 143-53. PMID: 17159247
4. [Expression of pituitary tumor-transforming gene in patients with multiple myeloma].  |  Wang, Z., et al. 2006. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 14: 1143-5. PMID: 17204181
5. PTTG expression in different experimental and human prolactinomas in relation to dopaminergic control of lactotropes.  |  Cristina, C., et al. 2007. Mol Cancer. 6: 4. PMID: 17222350
6. PTTG and PBF repress the human sodium iodide symporter.  |  Boelaert, K., et al. 2007. Oncogene. 26: 4344-56. PMID: 17297475
7. PTTG overexpression promotes lymph node metastasis in human esophageal squamous cell carcinoma.  |  Yan, S., et al. 2009. Cancer Res. 69: 3283-90. PMID: 19351864
8. PTTG acts as a STAT3 target gene for colorectal cancer cell growth and motility.  |  Zhou, C., et al. 2014. Oncogene. 33: 851-61. PMID: 23416975
9. PTTG has a Dual Role of Promotion-Inhibition in the Development of Pituitary Adenomas.  |  Xiong, Z., et al. 2019. Protein Pept Lett. 26: 800-818. PMID: 37020362
10. Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).  |  Yamamoto, A., et al. 1996. J Cell Biol. 133: 99-110. PMID: 8601617
11. hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms. Evidence for a transcriptional activation function of hPTTG.  |  Domínguez, A., et al. 1998. Oncogene. 17: 2187-93. PMID: 9811450
12. Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication.  |  Tóth, A., et al. 1999. Genes Dev. 13: 320-33. PMID: 9990856

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PTTG Antibody (DCS-280) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-PTTG Antibody (DCS-280) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PTTG Antibody (DCS-280) Specifications

Alternate Product Name: Securin

Product Clone Name: DCS-280

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: PTTG

Epitope Species: human

Product Reactivity/Detection: PTTG

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for PTTG Antibody (DCS-280): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin