PCB Antibody (H-2): sc-271493

PCB Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects pyruvate carboxylase (PCB) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PCB antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Pyruvate carboxylase plays a crucial role in gluconeogenesis and anaplerosis by catalyzing the conversion of pyruvate to oxaloacetate, a key intermediate in the citric acid cycle. This enzymatic activity is vital for maintaining glucose homeostasis and energy production, particularly in tissues such as the liver and brain, where PCB helps regulate metabolic pathways. The PCB gene is located on the long arm of chromosome 11, and mutations affecting PCB function can lead to serious metabolic disorders, including pyruvate carboxylase deficiency, which is characterized by lactic acidosis, hypoglycemia, and neurological impairments. PCB monoclonal antibody (H-2) accurately detects and quantifies this important enzyme, making this tool essential for researchers studying metabolic diseases and mitochondrial function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PCB Antibody (H-2) References:

1. Pyruvate carboxylase in genetic obesity.  |  Lynch, CJ., et al. 1992. Am J Physiol. 262: E608-18. PMID: 1375435
2. Intercellular metabolic compartmentation in the brain: past, present and future.  |  Hertz, L. 2004. Neurochem Int. 45: 285-96. PMID: 15145544
3. 13C NMR isotopomer analysis of anaplerotic pathways in INS-1 cells.  |  Cline, GW., et al. 2004. J Biol Chem. 279: 44370-5. PMID: 15304488
4. Transcarboxylase 5S structures: assembly and catalytic mechanism of a multienzyme complex subunit.  |  Hall, PR., et al. 2004. EMBO J. 23: 3621-31. PMID: 15329673
5. Hyperammonemia with citrullinemia.  |  Karnik, D., et al. 2004. Indian Pediatr. 41: 842-4. PMID: 15347874
6. Carbonic anhydrase inhibitors: inhibition of the membrane-bound human isozyme IV with anions.  |  Innocenti, A., et al. 2004. Bioorg Med Chem Lett. 14: 5769-73. PMID: 15501038
7. Biotin deficiency reduces expression of SLC19A3, a potential biotin transporter, in leukocytes from human blood.  |  Vlasova, TI., et al. 2005. J Nutr. 135: 42-7. PMID: 15623830
8. Molecular cloning of a cDNA for human pyruvate carboxylase. Structural relationship to other biotin-containing carboxylases and regulation of mRNA content in differentiating preadipocytes.  |  Freytag, SO. and Collier, KJ. 1984. J Biol Chem. 259: 12831-7. PMID: 6548474
9. cDNA cloning of human kidney pyruvate carboxylase.  |  MacKay, N., et al. 1994. Biochem Biophys Res Commun. 202: 1009-14. PMID: 8048912
10. Multidrug resistance P-glycoprotein monoclonal antibody JSB-1 crossreacts with pyruvate carboxylase.  |  Rao, VV., et al. 1995. J Histochem Cytochem. 43: 1187-92. PMID: 8537634
11. Disorders of pyruvate carboxylase and the pyruvate dehydrogenase complex.  |  Robinson, BH., et al. 1996. J Inherit Metab Dis. 19: 452-62. PMID: 8884569
12. Molecular characterization of pyruvate carboxylase deficiency in two consanguineous families.  |  Wexler, ID., et al. 1998. Pediatr Res. 43: 579-84. PMID: 9585002

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PCB Antibody (H-2) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-PCB Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PCB Antibody (H-2) Specifications

Alternate Product Name: PCB

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 954-985

Epitope Target: PCB

Epitope Species: human

Product Reactivity/Detection: PCB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for PCB Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin