patched/PTCH1 Antibody (E-7): sc-518046
- patched/PTCH1 Antibody (E-7) is a mouse monoclonal IgG1 provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1402-1429 within a C-terminal cytoplasmic domain of patched of human origin
- recommended for detection of patched of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for patched/PTCH1 Antibody (E-7) for WB applications. These reagents are now offered in bundles with patched/PTCH1 Antibody (E-7) (see ordering information below).
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Patched Antibody (E-7) is a mouse monoclonal IgG1 antibody that detects patched protein in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Patched, a critical component of the hedgehog signaling pathway, plays a vital role in regulating cell growth and differentiation during embryonic development. Anti-patched antibody (E-7) acts as a receptor for hedgehog ligands, thereby modulating the activity of downstream signaling pathways that influence cellular processes. Patched (E-7) monoclonal antibody maintains proper cellular communication; when patched is mutated or dysregulated, uncontrolled cell proliferation can occur and contribute to the development of various cancers, including basal cell carcinoma, which is the most prevalent form of skin cancer in the United States, affecting approximately 750,000 individuals annually. Additionally, patched interacts with other proteins in the hedgehog pathway, such as Sonic hedgehog and patched 2, further emphasizing patched′s role in cellular signaling and development. Anti-patched antibody (E-7) serves as an essential tool for researchers investigating the complexities of the hedgehog signaling pathway and its implications in cancer biology.
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patched/PTCH1 Antibody (E-7) References:
- Hedgehog induces opposite changes in turnover and subcellular localization of patched and smoothened. | Denef, N., et al. 2000. Cell. 102: 521-31. PMID: 10966113
- Mutations in PATCHED-1, the receptor for SONIC HEDGEHOG, are associated with holoprosencephaly. | Ming, JE., et al. 2002. Hum Genet. 110: 297-301. PMID: 11941477
- Inhibition of neuroepithelial patched-induced apoptosis by sonic hedgehog. | Thibert, C., et al. 2003. Science. 301: 843-6. PMID: 12907805
- Patched homologue 1 mutations in four Japanese families with basal cell nevus syndrome. | Matsuzawa, N., et al. 2006. J Clin Pathol. 59: 1084-6. PMID: 17021131
- The Hedgehog receptor Patched controls lymphoid lineage commitment. | Uhmann, A., et al. 2007. Blood. 110: 1814-23. PMID: 17536012
- Planarian Hedgehog/Patched establishes anterior-posterior polarity by regulating Wnt signaling. | Yazawa, S., et al. 2009. Proc Natl Acad Sci U S A. 106: 22329-34. PMID: 20018728
- The Hedgehog receptor patched functions in multidrug transport and chemotherapy resistance. | Bidet, M., et al. 2012. Mol Cancer Res. 10: 1496-508. PMID: 22752092
- Activation of Smurf E3 ligase promoted by smoothened regulates hedgehog signaling through targeting patched turnover. | Huang, S., et al. 2013. PLoS Biol. 11: e1001721. PMID: 24302888
- New mutations and an updated database for the patched-1 (PTCH1) gene. | Reinders, MG., et al. 2018. Mol Genet Genomic Med. 6: 409-415. PMID: 29575684
- Biochemical evidence that patched is the Hedgehog receptor. | Marigo, V., et al. 1996. Nature. 384: 176-9. PMID: 8906794
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
patched/PTCH1 Antibody (E-7) | sc-518046 | 200 µg/ml | $322.00 | |||
patched/PTCH1 Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-541119 | 200 µg Ab; 10 µg BP | $361.00 | |||
patched/PTCH1 Antibody (E-7): m-IgG1 BP-HRP Bundle | sc-542595 | 200 µg Ab; 20 µg BP | $361.00 |