PARD3B Antibody (F-12): sc-398887

PARD3B Antibody (F-12) is a mouse monoclonal IgG2a antibody that detects PARD3B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PARD3B, also known as par-3 partitioning defective 3 homolog B, plays a crucial role in maintaining cellular asymmetry, which is essential for proper development of multicellular organisms. This 1,205 amino acid protein functions as a putative adapter within the endomembrane system, facilitating cell polarization and asymmetric cell division. PARD3B is particularly important for the formation of epithelial tight junctions, as PARD3B localizes to cell junctions where PARD3B colocalizes with ZO-1 (zona occludens protein 1), thereby contributing to the integrity and functionality of epithelial barriers. PARD3B is expressed in various tissues, with highest levels found in skeletal muscle, lung, and kidney, while moderate expression is observed in pancreas, brain, heart, liver, and placenta. PARD3B exists in five alternatively spliced isoforms and contains three PDZ (DHR) domains, which are critical for protein-protein interactions. The gene encoding PARD3B is located on human chromosome 2q33.2, highlighting evolutionary significance and potential implications in developmental biology and disease processes. Anti-PARD3B antibody (F-12) is an invaluable tool for researchers investigating mechanisms of cell polarization and PARD3B′s role in various physiological and pathological contexts.

The preferred PARD3B antibody is PARD3B Antibody (E-9): sc-398761.