p-Stat3 Antibody (pS727.49): sc-136193

p-Stat3 Antibody (pS727.49) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 727 phosphorylated Stat3 isoform α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-Stat3 monoclonal antibody (pS727.49) is offered in a non-conjugated form, allowing for versatile experimental setups. Stat3 plays a crucial role in mediating cellular responses to various extracellular signals, particularly in inflammation and immune responses. Phosphorylation at Ser 727 is essential for full transcriptional activity of Stat3, complementing Tyr 705 phosphorylation by enhancing interactions with co-activators and transcriptional machinery. Ser 727 phosphorylation is often associated with metabolic stress and mitochondrial functions, indicating a role in non-canonical Stat3 signaling pathways. Ser 727 phosphorylation has been linked to fine-tuning Stat3 activity in response to specific stimuli, highlighting importance in regulating context-dependent gene expression. Stat3 is primarily located in cytoplasm and translocates to nucleus upon activation, where Stat3 regulates expression of genes involved in cell growth, survival, and differentiation. Cytoplasmic-nuclear shuttling of Stat3 allows for rapid responses to stimuli such as cytokines and growth factors. Dysregulation of Stat3 activity has been implicated in numerous diseases, including cancer, where persistent activation can lead to uncontrolled cell proliferation and survival. Understanding Stat3 localization and activation dynamics is essential for developing targeted therapies aimed at modulating activity in various pathological conditions. p-Stat3 monoclonal antibody (pS727.49) serves as an essential tool for studying phosphorylation-dependent activation of Stat3 at Ser 727 and roles in cellular signaling, gene regulation, and disease mechanisms.

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p-Stat3 Antibody (pS727.49) References:

1. Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis.  |  David, D., et al. 2011. J Exp Clin Cancer Res. 30: 56. PMID: 21575192
2. p-Stat3 and bcr/abl gene expression in chronic myeloid leukemia and their relation to imatinib therapy.  |  Sayed, D., et al. 2014. Leuk Res. 38: 243-50. PMID: 24374144
3. Phosphorylated STAT3 physically interacts with NPM and transcriptionally enhances its expression in cancer.  |  Ren, Z., et al. 2015. Oncogene. 34: 1650-7. PMID: 24793791
4. Clinical implications of phosphorylated STAT3 expression in De Novo diffuse large B-cell lymphoma.  |  Ok, CY., et al. 2014. Clin Cancer Res. 20: 5113-23. PMID: 25124685
5. IL-6, through p-STAT3 rather than p-STAT1, activates hepatocarcinogenesis and affects survival of hepatocellular carcinoma patients: a cohort study.  |  Kao, JT., et al. 2015. BMC Gastroenterol. 15: 50. PMID: 25908103
6. EGFR and KRAS mutations do not enrich for the activation of IL-6, JAK1 or phosphorylated STAT3 in resected lung adenocarcinoma.  |  Clay, TD., et al. 2017. Med Oncol. 34: 175. PMID: 28879441
7. Clinicopathological significance and prognostic role of p-STAT3 in patients with hepatocellular carcinoma.  |  Liang, C., et al. 2018. Onco Targets Ther. 11: 1203-1214. PMID: 29551899
8. Exosomal transfer of p-STAT3 promotes acquired 5-FU resistance in colorectal cancer cells.  |  Zhang, Q., et al. 2019. J Exp Clin Cancer Res. 38: 320. PMID: 31324203
9. Enhanced Hsa-miR-181d/p-STAT3 and Hsa-miR-181d/p-STAT5A Ratios Mediate the Anticancer Effect of Garcinol in STAT3/5A-Addicted Glioblastoma.  |  Liu, HW., et al. 2019. Cancers (Basel). 11: PMID: 31783691
10. Malat1 regulates PMN-MDSC expansion and immunosuppression through p-STAT3 ubiquitination in sepsis.  |  Wang, Y., et al. 2024. Int J Biol Sci. 20: 1529-1546. PMID: 38385073

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Stat3 Antibody (pS727.49) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Stat3 Antibody (pS727.49) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Stat3 Antibody (pS727.49) Specifications

Alternate Product Name: Stat3

Product Clone Name: pS727.49

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 727

Epitope Target: Stat3

Epitope Species: human

Product Reactivity/Detection: Ser 727 phosphorylated Stat3 isoform α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for p-Stat3 Antibody (pS727.49): N/A

The preferred p-Stat3 antibody is p-Stat3 Antibody (B-7): sc-8059.