Anticorpo p-Stat1 (12C5) raccomandato per il rilevamento di Ser 727 phosphorylated Stat1 di origine human in WB e IP

Anticorpo p-Stat1 (12C5): sc-81522

Anticorpo p-Stat1 (12C5) valutazione 4.0 di 5 di 2.
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Schede tecniche

    • p-Stat1 Antibody 12C5 è un monoclonale di topo IgG1, citato in 3 pubblicazioni, fornito in 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide corresponding to amino acids Ser 727 of Stat1 of human origin
    • raccomandato per il rilevamento di Ser 727 phosphorylated Stat1 di origine human in WB e IP
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Stat1 Antibody (12C5). This work is in progress.
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I am using p-Stat1 (12C5): sc-81522 antibody for immunoprecipitation, how much antibody should I use?

Richiesta da: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Risposta da: Technical Support
Data di pubblicazione: 2017-03-24
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Rated 4 di 5 di da WB publication in human CD4+ T cells WB publication in human CD4+ T cells. -SCBT Publication Review
Data di pubblicazione: 2015-03-18
Rated 4 di 5 di da Working well in Western Blot in EGF stimulated Working well in Western Blot in EGF stimulated and pervanadate treated A549 whole cell lysates. -SCBT QC
Data di pubblicazione: 2014-10-15
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