Anticorpo p-Rb (A-5) raccomandato per il rilevamento di Ser 249 and Thr 252 dually-phosphorylated Rb di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo p-Rb (A-5): sc-377528

Anticorpo p-Rb (A-5) valutazione 3.9 di 5 di 7.
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Schede tecniche

    • p-Rb Antibody A-5 è un monoclonale di topo IgG2b κ, citato in 14 pubblicazioni, fornito in 200 µg/ml
    • p-Rb Antibody (A-5) é raccomandato per il rilevamento di Ser 249 and Thr 252 dually-phosphorylated Rb di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • p-Rb Antibody (A-5) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di p-Rb (A-5): sc-377528.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for p-Rb Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Rb Antibody (A-5) (see ordering information below).
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Could you please provide the concentration of the glue of WB, the membrane transfer conditions, the type of sealing liquid and the sealing time, and the primary concentration?

Richiesta da: lily 12
Thank you for your question. For the transfer conditions, please reach out to the manufacturer of your transfer equipment. The recommended starting dilution of this primary antibody is 1:50 dilution. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Tech Service
Data di pubblicazione: 2019-09-06

What application is the blocking peptide sc-377528 P appropriate for?

Richiesta da: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 1 di 5 di da Didnot work for WB did not work for western - samples were human cells in culture
Data di pubblicazione: 2017-11-21
Rated 3 di 5 di da not good for fish protein some unspecific bands showed when do the fish experiment.
Data di pubblicazione: 2017-08-08
Rated 5 di 5 di da perfect antibody. I applied sc-377528 sample half a month ago to test the changes of p-Rb in my cell after treatment and happy to find a good result.
Data di pubblicazione: 2017-08-08
Rated 4 di 5 di da very good there was three bands in turbot, primary antibody 1:200, second antibody 1:10000. good enough
Data di pubblicazione: 2017-06-20
Rated 4 di 5 di da Fairly potent and specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is recommended for Western blotting. There was medium detection of a ~105 kDa target-sized protein in overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and only significant cross-reactivity with ~45 kDa protein.
Data di pubblicazione: 2016-12-04
Rated 5 di 5 di da Good bands in Western Blot acquired Good bands in Western Blot acquired. -SCBT QC
Data di pubblicazione: 2015-05-01
Rated 5 di 5 di da Beautiful immunoperoxidase nuclear and cytoplasmic Beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human vagina tissue. -SCBT QC
Data di pubblicazione: 2015-03-22
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