Anticorpo p-GSK-3 alpha / beta (6D3) raccomandato per il rilevamento di Tyr 279 phosphorylated GSK-3α and Tyr 216 phosphorylated GSK-3β di origine mouse, rat e human in WB e IP

Anticorpo p-GSK-3α/β (6D3): sc-81496

Anticorpo p-GSK-3α/β (6D3) valutazione 4.4 di 5 di 11.
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Schede tecniche

    • p-GSK-3 alpha / beta Antibody 6D3 è un monoclonale di topo IgG1, citato in 21 pubblicazioni, fornito in 50 µg/0.5 ml
    • raised against phosphopeptide corresponding to amino acid residues surrounding tyrosine 279 of GSK-3α and tyrosine 216 of GSK-3β of human origin
    • raccomandato per il rilevamento di Tyr 279 phosphorylated GSK-3α and Tyr 216 phosphorylated GSK-3β di origine mouse, rat e human in WB e IP
    • Contattaci per ricevere un FREE 10 µg sample di p-GSK-3α/β (6D3): sc-81496.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-GSK-3α/β Antibody (6D3) for WB applications. These reagents are now offered in bundles with p-GSK-3α/β Antibody (6D3) (see ordering information below).
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I am using p-GSK-3α/β (6D3): sc-81496 antibody for immunoprecipitation, how much antibody should I use?

Richiesta da: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Risposta da: Technical Support
Data di pubblicazione: 2017-03-24
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Rated 5 di 5 di da It really works well!! I purchased and tested this product using western blots (1:500 dilution, sample: MIN6 cells), showing that their bands are clear.
Data di pubblicazione: 2020-06-12
Rated 5 di 5 di da Band is very clean We performed whole cell lysate and used this antibody to test for phosphorylated GSK3a/b. It's very good.
Data di pubblicazione: 2018-11-28
Rated 4 di 5 di da Worked well! Worked well when probing mouse lysates at 1:200 dilution via WB. Gave nice bands after longer exposure times.
Data di pubblicazione: 2018-03-23
Rated 4 di 5 di da Good antibody I tested the antibody for endogenous pGSK3. It worked well.
Data di pubblicazione: 2018-02-26
Rated 4 di 5 di da 功能可以 我是3个月前申请的小样产品,首先就是客服很周到,很贴心及时。在收到产品后,并没有马上做实验,是后面才用的,所有评论比较晚,但是产品功能可以,很满意。
Data di pubblicazione: 2018-02-15
Rated 5 di 5 di da Anticorps Super specifique ! J'ai testé cet anticorps au 1/500 en WB et il marche super bien, propre et sans bruit de fond !
Data di pubblicazione: 2017-12-14
Rated 4 di 5 di da Good antibody good antibody shows perfect bands, two close very sharp bands on my healthy human liver lysate
Data di pubblicazione: 2017-12-04
Rated 5 di 5 di da Good antibody for WB The p-GSK-3 alpha / beta Antibody (6D3): sc-81496 works well for detection via Western blot applications. This antibody has also been cited several times for further verification.
Data di pubblicazione: 2017-02-03
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