Anticorpo Oas1a (E-2): sc-365072

Oas1a Antibody (E-2) is an IgG2a κ mouse monoclonal Oas1a antibody suitable for the detection of Oas1a of mouse and rat origin. Oas1a Antibody (E-2) is available as the non-conjugated anti-Oas1a antibody form, as well as multiple conjugated forms of anti-Oas1a antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The 2′-, 5′- oligoadenylate synthetases (OASs) are interferon-induced proteins that play a putative role in mediating resistance to virus infection, control of cell growth, differentiation and apoptosis. OAS1, which functions as a homotetramer, is characterized by its capacity to catalyze the synthesis of 2′-, 5′- oligomers of adenosine (2-5As). OAS1 binds double-stranded RNA and polymerizes ATP into PPP(A2′P5′A)N oligomers, activating latent RNase L which, when activated, cleaves single-stranded RNAs. This RNase L activity leads to the inhibition of cellular protein synthesis and the impairment of viral replication. OAS1, a 400 amino acid containing protein, is also important in evaluating the interferon response in RNAi studies, and is implicated in diabetes mellitus susceptibility. Oas1a is one of the known rodent homologs of human OAS1, which are thought to mediate cell growth, differentiation and apoptosis, as well as host resistance to viral infection.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. A specific isozyme of 2′-5′ oligoadenylate synthetase is a dual function proapoptotic protein of the Bcl-2 family.  |  Ghosh, A., et al. 2001. J Biol Chem. 276: 25447-55. PMID: 11323417
2. Characterization of the 2′-5′-oligoadenylate synthetase ubiquitin-like family.  |  Eskildsen, S., et al. 2003. Nucleic Acids Res. 31: 3166-73. PMID: 12799444
3. Variation in antiviral 2′,5′-oligoadenylate synthetase (2′5′AS) enzyme activity is controlled by a single-nucleotide polymorphism at a splice-acceptor site in the OAS1 gene.  |  Bonnevie-Nielsen, V., et al. 2005. Am J Hum Genet. 76: 623-33. PMID: 15732009
4. Polymorphisms of interferon-inducible genes OAS-1 and MxA associated with SARS in the Vietnamese population.  |  Hamano, E., et al. 2005. Biochem Biophys Res Commun. 329: 1234-9. PMID: 15766558
5. OAS1 splice site polymorphism controlling antiviral enzyme activity influences susceptibility to type 1 diabetes.  |  Field, LL., et al. 2005. Diabetes. 54: 1588-91. PMID: 15855350
6. Enhancing and confirming the specificity of RNAi experiments.  |  Cullen, BR. 2006. Nat Methods. 3: 677-81. PMID: 16929311
7. Structure of two forms of the interferon-induced (2′-5′) oligo A synthetase of human cells based on cDNAs and gene sequences.  |  Benech, P., et al. 1985. EMBO J. 4: 2249-56. PMID: 2416561
8. Induction of 2.5 OAS gene expression and activity is not sufficient for IFN-gamma-induced neuroblastoma cell differentiation.  |  Corrias, MV., et al. 1995. Int J Cancer. 62: 223-9. PMID: 7622300
9. The human 2′,5′-oligoadenylate synthetase locus is composed of three distinct genes clustered on chromosome 12q24.2 encoding the 100-, 69-, and 40-kDa forms.  |  Hovnanian, A., et al. 1998. Genomics. 52: 267-77. PMID: 9790745