ErbB2/HER2 Antibody (3B5): sc-33684

Neu Antibody (3B5) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Neu protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). anti-Neu antibody (3B5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neu, also known as ErbB-2 or HER2, is a member of the epidermal growth factor receptor (EGFR) family, which consists of receptor tyrosine kinases that play critical roles in cell signaling pathways related to growth and differentiation. Neu is primarily located on the cell membrane, where binding with ligands can form homodimers or heterodimers with other EGFR family members, such as ErbB-3. This dimerization activates downstream signaling cascades, including the phosphoinositide 3-kinase pathway, which regulates cell proliferation, survival, and motility. Aberrant expression of Neu is associated with various cancers, particularly breast carcinoma, where amplification or overexpression occurs in 20-30% of cases, making Neu a significant biomarker for disease prognosis and a potential target for therapeutic interventions. Neu monoclonal antibody (3B5) enables researchers to gain valuable insights into Neu′s role in cancer biology and therapeutic potential.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ErbB2/HER2 Antibody (3B5) has 73 citations in a variety of scientific publications.

Primary antibodies like Anti-ErbB2/HER2 Antibody (3B5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ErbB2/HER2 Antibody (3B5) Specifications

Alternate Product Name: HER2

Product Clone Name: 3B5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1242-1255

Epitope Target: Neu

Epitope Species: human

Product Reactivity/Detection: Neu

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 73 citations in various scientific publications

Available Conjugates/Forms for ErbB2/HER2 Antibody (3B5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred antibody is Antibody (): sc-8.