MyD88 Antibody (E-11): sc-74532

MyD88 Antibody (E-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects MyD88 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-MyD88 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MyD88 plays a crucial role in the innate immune response, acting as an adaptor protein that facilitates the signaling cascade initiated by the interleukin-1 receptor, which is vital for the activation of the NFκB pathway. This pathway is essential for the transcription of pro-inflammatory cytokines and other immune response genes, highlighting MyD88′s importance in mediating inflammatory responses and maintaining immune homeostasis. MyD88′s structure includes a distinctive N-terminal death domain, which is critical for interaction with interleukin-1 receptor-associated kinase (IRAK), and a Toll/IL-1R homology domain that enables association with the IL-1 receptor complex. These structural features are fundamental for protein-protein interactions that drive downstream signaling necessary for effective immune responses, making MyD88 a key player in the body′s defense mechanisms against pathogens.

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MyD88 Antibody (E-11) References:

1. Oncogenically active MYD88 mutations in human lymphoma.  |  Ngo, VN., et al. 2011. Nature. 470: 115-9. PMID: 21179087
2. MYD88 L265P somatic mutation in Waldenström's macroglobulinemia.  |  Treon, SP., et al. 2012. N Engl J Med. 367: 826-33. PMID: 22931316
3. The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways.  |  Pereira, M., et al. 2022. Cell Rep. 40: 111225. PMID: 35977521
4. Schisandrin B Attenuates Diabetic Cardiomyopathy by Targeting MyD88 and Inhibiting MyD88-Dependent Inflammation.  |  Luo, W., et al. 2022. Adv Sci (Weinh). 9: e2202590. PMID: 36180407
5. Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infection.  |  Yoshimoto, T., et al. 2022. Nat Commun. 13: 6648. PMID: 36333322
6. Interaction of the pelle kinase with the membrane-associated protein tube is required for transduction of the dorsoventral signal in Drosophila embryos.  |  Galindo, RL., et al. 1995. Development. 121: 2209-18. PMID: 7635064
7. Molecular characterization and modular analysis of human MyD88.  |  Hardiman, G., et al. 1996. Oncogene. 13: 2467-75. PMID: 8957090
8. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.  |  Muzio, M., et al. 1997. Science. 278: 1612-5. PMID: 9374458
9. MyD88: an adapter that recruits IRAK to the IL-1 receptor complex.  |  Wesche, H., et al. 1997. Immunity. 7: 837-47. PMID: 9430229
10. MyD88, an adapter protein involved in interleukin-1 signaling.  |  Burns, K., et al. 1998. J Biol Chem. 273: 12203-9. PMID: 9575168
11. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function.  |  Adachi, O., et al. 1998. Immunity. 9: 143-50. PMID: 9697844
12. MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways.  |  Medzhitov, R., et al. 1998. Mol Cell. 2: 253-8. PMID: 9734363

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MyD88 Antibody (E-11) has 124 citations in a variety of scientific publications.

Primary antibodies like Anti-MyD88 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MyD88 Antibody (E-11) Specifications

Alternate Product Name: MYD88

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-296

Epitope Target: MyD88

Epitope Species: human

Product Reactivity/Detection: MyD88

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 124 citations in various scientific publications

Available Conjugates/Forms for MyD88 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MyD88 antibody is MyD88 Antibody (B-1): sc-136970.