LAMP-2 Antibody (M3/84): sc-19991

LAMP-2 Antibody (M3/84) is a rat monoclonal IgG1 antibody targeting an epitope mapped to an immunoadsorbent-purified mouse macrophage glycoprotein fraction, with species reactivity in mouse, rat, and human. LAMP-2 monoclonal antibody (M3/84) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). LAMP-2, also known as CD107B, is a glycosylated type I transmembrane protein localized to the lysosomal membrane. The structure includes an extracellular domain rich in N-linked glycosylation, which is crucial for maintaining lysosomal membrane integrity, and a short cytoplasmic tail that interacts with intracellular trafficking machinery. Glycosylation of LAMP-2 contributes to stability, protection of the lysosomal membrane from hydrolytic enzymes, and plays a role in autophagy by facilitating the fusion of autophagosomes with lysosomes. LAMP-2 is sorted at the trans-Golgi network and transported via specialized vesicular pathways, underscoring importance in cellular homeostasis and macromolecule degradation. Alterations in LAMP-2 structure or expression are associated with lysosomal storage disorders like Danon disease and may influence tumor progression through impacts on cellular adhesion and metastasis. LAMP-2 (M3/84) antibody serves as a valuable tool for studying structural and functional roles in lysosomal biology and disease mechanisms.

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LAMP-2 Antibody (M3/84) References:

1. Disturbed cholesterol traffic but normal proteolytic function in LAMP-1/LAMP-2 double-deficient fibroblasts.  |  Eskelinen, EL., et al. 2004. Mol Biol Cell. 15: 3132-45. PMID: 15121881
2. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.  |  Grützkau, A., et al. 2004. Cytometry A. 61: 62-8. PMID: 15351990
3. LAMP-2 deficient mice show depressed cardiac contractile function without significant changes in calcium handling.  |  Stypmann, J., et al. 2006. Basic Res Cardiol. 101: 281-91. PMID: 16604439
4. Role for LAMP-2 in endosomal cholesterol transport.  |  Schneede, A., et al. 2011. J Cell Mol Med. 15: 280-95. PMID: 19929948
5. LAMP-2-deficient human B cells exhibit altered MHC class II presentation of exogenous antigens.  |  Crotzer, VL., et al. 2010. Immunology. 131: 318-30. PMID: 20518820
6. LAMP-2 gene expression in peripheral leukocytes is increased in patients with coronary artery disease.  |  Wu, G., et al. 2011. Clin Cardiol. 34: 239-43. PMID: 21462217
7. LAMP-2 deficiency leads to hippocampal dysfunction but normal clearance of neuronal substrates of chaperone-mediated autophagy in a mouse model for Danon disease.  |  Rothaug, M., et al. 2015. Acta Neuropathol Commun. 3: 6. PMID: 25637286
8. A decline of LAMP- 2 predicts ursodeoxycholic acid response in primary biliary cirrhosis.  |  Wang, L., et al. 2015. Sci Rep. 5: 9772. PMID: 25894308
9. Host cell protein LAMP-2 is the receptor for Trypanosoma cruzi surface molecule gp82 that mediates invasion.  |  Rodrigues, JPF., et al. 2019. Cell Microbiol. 21: e13003. PMID: 30609224
10. Comparative study of the steady-state subcellular distribution of lysosome-associated membrane glycoprotein-2 (LAMP-2) isoforms with GYXXΦ-type tyrosine-based motifs that interact differently with four adaptor protein (AP) complexes.  |  Yamaguchi, F., et al. 2024. J Biochem. 175: 275-287. PMID: 37983719

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LAMP-2 Antibody (M3/84) has 60 citations in a variety of scientific publications.

Primary antibodies like Anti-LAMP-2 Antibody (M3/84) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LAMP-2 Antibody (M3/84) Specifications

Alternate Product Name: LAMP2

Product Clone Name: M3/84

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: LAMP-2

Epitope Species: mouse

Product Reactivity/Detection: LAMP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 60 citations in various scientific publications

Available Conjugates/Forms for LAMP-2 Antibody (M3/84): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred LAMP-2 antibody is LAMP-2 Antibody (H4B4): sc-18822.