Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KCNQ1 Double Nickase Plasmid (h): sc-400933-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KCNQ1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KCNQ1 Double Nickase Plasmid (h) and KCNQ1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KCNQ1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KCNQ1 Antibody (G-8): sc-365186
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCNQ1 Double Nickase Plasmid (h) | sc-400933-NIC | 20 µg | $410.00 | |||
KCNQ1 Double Nickase Plasmid (h2) | sc-400933-NIC-2 | 20 µg | $410.00 |
KCNQ1 encodes a voltage-gated potassium channel α-subunit that assembles as a tetramer and, in many tissues, partners with KCNE family β-subunits to shape membrane repolarization. In excitable cells, KCNQ1-mediated currents contribute to action potential termination and electrical stability, while in epithelia the channel helps regulate ion transport and fluid homeostasis. Channel activity is modulated by signaling inputs such as cAMP/PKA-dependent phosphorylation and membrane phosphoinositides, linking KCNQ1 to broader control of cellular excitability and transport processes. Genetic and epigenetic alterations in KCNQ1 have been associated with cardiac arrhythmia phenotypes and imprinted-locus dysregulation, supporting its relevance for mechanistic studies of electrophysiology and gene regulation.
KCNQ1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KCNQ1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KCNQ1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KCNQ1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KCNQ1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.