ISYNA1 Double Nickase Plasmid (h): sc-404785-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ISYNA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ISYNA1 Double Nickase Plasmid (h) and ISYNA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ISYNA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ISYNA1 Antibody (C-9): sc-271830
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ISYNA1 Double Nickase Plasmid (h) | sc-404785-NIC | 20 µg | $410.00 | |||
ISYNA1 Double Nickase Plasmid (h2) | sc-404785-NIC-2 | 20 µg | $410.00 |
ISYNA1 encodes myo-inositol-3-phosphate synthase 1, the rate-limiting enzyme that converts glucose-6-phosphate to inositol-3-phosphate, initiating de novo myo-inositol biosynthesis. This activity supplies inositol for phosphatidylinositol and inositol phosphate production, supporting membrane biogenesis and PI3K/AKT-linked signaling dynamics, vesicular trafficking, and osmotic homeostasis. ISYNA1-dependent inositol metabolism contributes to cellular stress adaptation and influences lipid and carbohydrate metabolic programs. Dysregulation of inositol and phosphoinositide pathways is implicated in neurobiology and metabolic phenotypes, making ISYNA1 a useful node for mechanistic studies of signaling and membrane-associated processes.
ISYNA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ISYNA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ISYNA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ISYNA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ISYNA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.