Anticorpo IL-7 (D-9) raccomandato per il rilevamento di IL-7 di origine human in WB, IP, IF, IHC(P) e ELISA

Anticorpo IL-7 (D-9): sc-365306

Anticorpo IL-7 (D-9) valutazione 5.0 di 5 di 4.
  • y_2022, m_1, d_26, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_it_IT, sid_365306, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 124ms
  • REVIEWS, PRODUCT
loading
5
4
4
0
3
0
2
0
1
0

Schede tecniche

    • Anti-IL-7 Antibody D-9 è un monoclonale di topo IgG1 κ IL-7 antibody, citato in 2 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 27-177 di IL-7 di origine human
    • IL-7 Antibody (D-9) é raccomandato per il rilevamento di IL-7 di origine human in WB, IP, IF, IHC(P) e ELISA
    • IL-7 Antibody (D-9) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di IL-7 (D-9): sc-365306.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for IL-7 Antibody (D-9) for WB and IHC(P) applications. These reagents are now offered in bundles with IL-7 Antibody (D-9) (see ordering information below).
loading

Hello, kindly I would like to know what does it mean "fusion protein" why in WB we can identifies 3 bands from different molecular weight? are those bands IL-7 ? Thanks a lot

Richiesta da: Carlos99
Thank you for your question. On this antibody's product page we show a western blot image of full length human recombinant IL-7 fusion protein. This protein consists of a peptide corresponding to amino acids 27-177 of IL-7 of human origin, and several different marker proteins. In western blots with regular cell lysates the expected molecular weight is 25 kDa.
Risposta da: Tech Support Europe
Data di pubblicazione: 2020-06-15

hi is the antibody can used for detection of IL7 from mouse origin?

Richiesta da: ali a
Thank you for your question. We have not yet tested this antibody in mouse samples, and so it is not a recommended species at this time.
Risposta da: Technical Support
Data di pubblicazione: 2019-05-01

Can this antibody recognize murine IL-7? specifically, detection via IHC or IF? Thanks in advance!

Richiesta da: katinthehat
Thank you for your question. This antibody is currently only recommended for human use, but it has not been tested on mouse samples. It may work, but can not be recommended for that use. We would be happy to send you a sample of sc-365306 if you wish to try it. Please reach out to Technical support at [email protected], or via chat directly on our website, www.scbt.com, and we would be happy to supply you with a sample.
Risposta da: Tech Service
Data di pubblicazione: 2017-12-04

I am using IL-7 (D-9): sc-365306 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Richiesta da: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
  • y_2022, m_1, d_26, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_it_IT, sid_365306, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 142ms
  • QUESTIONS, PRODUCT
Rated 5 di 5 di da Great IF staining results We used this antibody to take IF pictures and got very nice images from the binding in HeLa cells
Data di pubblicazione: 2017-01-27
Rated 5 di 5 di da Good publishable data in WB Good publishable data in WB, Flow and ChIP in A549 and LH7 cell. -SCBT Publication Review
Data di pubblicazione: 2015-04-27
Rated 5 di 5 di da Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Data di pubblicazione: 2015-01-10
Rated 5 di 5 di da Robust signal in WB in human recombinant IL Robust signal in WB in human recombinant IL-7 fusion protein. -SCBT QC
Data di pubblicazione: 2015-01-07
  • y_2022, m_1, d_26, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_it_IT, sid_365306, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT