Iba1 Antibody (1022-5): sc-32725

Iba1 Antibody (1022-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Iba1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). Iba1 (1022-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Iba1 protein, also known as ionized calcium-binding adapter molecule 1 or allograft inflammatory factor-1, is a 147 amino acid cytoplasmic protein that plays a crucial role in macrophage activation and function, making Iba1 significant in immune responses and neuroinflammation. Structurally, Iba1 contains two EF-hand domains that facilitate calcium binding, which is essential for function in cellular signaling pathways. Upon stimulation by cytokines and interferons, Iba1 translocates from initial colocalization with actin to lamellipodia, highlighting a dynamic role in cellular responses to injury. Additionally, Iba1 serves as a marker for human microglia and is expressed by macrophages in injured skeletal muscle, indicating importance in both the central nervous system and peripheral tissues. The gene encoding Iba1 is located on chromosome 6p21.33 within the tumor necrosis factor gene cluster, which is part of the human major histocompatibility complex region, further underscoring relevance in immune regulation and disease processes.

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Iba1 Antibody (1022-5) References:

1. A new member of the Ig superfamily and a V-ATPase G subunit are among the predicted products of novel genes close to the TNF locus in the human MHC.  |  Neville, MJ. and Campbell, RD. 1999. J Immunol. 162: 4745-54. PMID: 10202016
2. AIF-1 is an actin-polymerizing and Rac1-activating protein that promotes vascular smooth muscle cell migration.  |  Autieri, MV., et al. 2003. Circ Res. 92: 1107-14. PMID: 12714565
3. Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity.  |  Ohsawa, K., et al. 2004. J Neurochem. 88: 844-56. PMID: 14756805
4. High-Density SNP genotyping defines 17 distinct haplotypes of the TNF block in the Caucasian population: implications for haplotype tagging.  |  Allcock, RJ., et al. 2004. Hum Mutat. 24: 517-25. PMID: 15523649
5. Increased smooth muscle cell activation and neointima formation in response to injury in AIF-1 transgenic mice.  |  Sommerville, LJ., et al. 2008. Arterioscler Thromb Vasc Biol. 28: 47-53. PMID: 17991871
6. Allograft inflammatory factor-1 augments macrophage phagocytotic activity and accelerates the progression of atherosclerosis in ApoE-/- mice.  |  Mishima, T., et al. 2008. Int J Mol Med. 21: 181-7. PMID: 18204784
7. AIF-1 expression regulates endothelial cell activation, signal transduction, and vasculogenesis.  |  Tian, Y., et al. 2009. Am J Physiol Cell Physiol. 296: C256-66. PMID: 18787073
8. Staining of HLA-DR, Iba1 and CD68 in human microglia reveals partially overlapping expression depending on cellular morphology and pathology.  |  Hendrickx, DAE., et al. 2017. J Neuroimmunol. 309: 12-22. PMID: 28601280
9. Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain.  |  González Ibanez, F., et al. 2019. J Vis Exp.. PMID: 31710033
10. Co-expression patterns of microglia markers Iba1, TMEM119 and P2RY12 in Alzheimer's disease.  |  Kenkhuis, B., et al. 2022. Neurobiol Dis. 167: 105684. PMID: 35247551
11. cDNA cloning of human allograft inflammatory factor-1: tissue distribution, cytokine induction, and mRNA expression in injured rat carotid arteries.  |  Autieri, MV. 1996. Biochem Biophys Res Commun. 228: 29-37. PMID: 8912632
12. IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein.  |  Autieri, MV. and Agrawal, N. 1998. J Biol Chem. 273: 14731-7. PMID: 9614071

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Iba1 Antibody (1022-5) has 225 citations in a variety of scientific publications.

Primary antibodies like Anti-Iba1 Antibody (1022-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Iba1 Antibody (1022-5) Specifications

Alternate Product Name: Iba1

Product Clone Name: 1022-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Iba1

Epitope Species: human

Product Reactivity/Detection: Iba1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 225 citations in various scientific publications

Available Conjugates/Forms for Iba1 Antibody (1022-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin