Anticorpo HSP 56 (329.1) raccomandato per il rilevamento di HSP 56 di origine mouse, rat e human in WB, IP e ELISA

Anticorpo HSP 56 (329.1): sc-100758

Anticorpo HSP 56 (329.1) valutazione 3.2 di 5 di 5.
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Schede tecniche

    • Anti-HSP 56 Antibody 329.1 è un monoclonale di topo IgG2a κ HSP 56 antibody, citato in 3 pubblicazioni, fornito in 100 µg/ml
    • generato contro il ricombinante HSP 56 di origine human
    • raccomandato per il rilevamento di HSP 56 di origine mouse, rat e human in WB, IP e ELISA
    • Contattaci per ricevere un FREE 10 µg sample di HSP 56 (329.1): sc-100758.

Why does one blot show no band for HSP56 in untransfected 293T cells the other blot does, and why don't you see more of it when over expressed? These data do not convince me that this antibody is actually staining for HSP 56

Richiesta da: real or not
Thank you for your question. HSP proteins are generally pretty ubiquitously expressed so it is harder to show upregulation. The differences in signal observed between untransfected control 293T and HSP 56 transfected 293T could be a result of differences in protein loading, lysate preparations, exposure times, buffer quality, etc. There are many variables that can contribute to this observable difference. The data we show on our website is intended to be indicative of what you would expect when using this antibody. Please contact our Technical Service Department for further support.
Risposta da: Technical Support
Data di pubblicazione: 2019-03-22

What is the molecular weight of HSP 56 ?

Richiesta da: TinTin
Thank you for your question. HSP 56 has an expected molecular weight of approximately 56 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-27
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Rated 2 di 5 di da Use to detect HSP56 protein levels This antibody is very useful for protein detection in animal and human cell compartments. In particular, we used this antibody to detect protein levels in nuclear and cytosolic cell compartments in hippocampus and prefrontal cortex of mice and rats. In human samples we did not obtained protein bands detecting HSP56 levels. In general, we are not very satisfied with the obtained results, because Ab displayed low purity and high background in Western blot. Dilution to 1:200 for Western blot.
Data di pubblicazione: 2017-11-23
Rated 2 di 5 di da Use to detect HSP56 protein levels This antibody is very useful for protein detection in animal and human cell compartments. In particular, we used this antibody to detect protein levels in nuclear and cytosolic cell compartments in hippocampus and prefrontal cortex of mice and rats. In human samples we did not obtained protein bands detecting HSP56 levels. In general, we are not very satisfied with the obtained results, because Ab displayed low purity and high background in Western blot. Dilution to 1:200 for Western blot.
Data di pubblicazione: 2017-11-23
Rated 4 di 5 di da No band detected in U87 cell lysates (WB) No band detected for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution.
Data di pubblicazione: 2017-09-10
Rated 4 di 5 di da Western blot analysis supporting specificity Western blot analysis supporting specificity of the antibody via of HSP 56 expression in non-transfected: sc-117752 and mouse HSP 56 transfected: sc-110358 293T whole cell lysates. -SCBT QC
Data di pubblicazione: 2015-03-30
Rated 4 di 5 di da Supporting western blot analysis of HSP 56 Supporting western blot analysis of HSP 56 expression in HeLa whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-02-28
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