GPR39 CRISPR/Cas9 KO Plasmid (m): sc-427882
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR39 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR39 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR39 CRISPR/Cas9 KO Plasmid (m) | sc-427882 | 20 µg | $397.00 |
Overview
Gpr39 encodes GPR39, a zinc-sensing G protein-coupled receptor implicated in regulating intracellular signaling cascades that shape cell survival, secretion, and inflammatory tone. In mouse systems, GPR39 activity has been linked to modulation of cAMP- and Ca2+-dependent pathways and downstream kinase signaling, influencing epithelial barrier function, neuronal signaling, and metabolic homeostasis. Altered GPR39 signaling has been studied in contexts including metabolic dysfunction, gastrointestinal inflammation, and neurobehavioral phenotypes, making it a useful node for pathway interrogation. As a membrane receptor integrating extracellular cues with transcriptional and metabolic outputs, GPR39 provides a tractable target for dissecting GPCR-driven networks in vivo and in cultured cells.
GPR39 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gpr39 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gpr39 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gpr39 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR39 protein expression.
This CRISPR knockout system enables efficient generation of Gpr39-deficient cell models for investigation of GPR39 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gpr39 exon(s) critical for GPR39 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gpr39 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR39 CRISPR/Cas9 KO Plasmid (m) and GPR39 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gpr39 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR39 HDR Plasmid (m) and GPR39 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gpr39 homology arms to support homology-directed repair at defined Gpr39 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.