frizzled-6 Antibody (C-12): sc-393791

frizzled-6 Antibody (C-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects frizzled-6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-frizzled-6 antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. frizzled-6, also known as frizzled homolog 6 in Drosophila, plays a crucial role as a seven-transmembrane domain receptor that binds Wnt ligands, which are essential for regulating cell signaling pathways involved in development and tissue homeostasis. frizzled-6 is particularly important in influencing macroscopic hair patterning and other tissue polarity events, which are vital for proper organ formation and function. frizzled-6 contains a signal peptide and a cysteine-rich domain in its N-terminal extracellular region, but notably lacks a C-terminal PDZ domain-binding motif, which differentiates frizzled-6 from other frizzled family members. frizzled-6 interacts with frizzled-3 to influence neural tube closure and the orientation of hair bundles in auditory and vestibular sensory cells, highlighting frizzled-6′s significance in sensory organ development. In vitro studies using Madin-Darby canine kidney (MDCK) cells have shown that frizzled-6 forms a complex with Wnt-4 through its cysteine-rich domain, further underscoring frizzled-6′s role in cellular signaling. frizzled-6 expression is observed as a 4.4 kb mRNA in various human tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine, and colon, indicating frizzled-6′s widespread importance in human physiology.

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frizzled-6 Antibody (C-12) References:

1. The human Frizzled 6 (HFz6) acts as a negative regulator of the canonical Wnt. beta-catenin signaling cascade.  |  Golan, T., et al. 2004. J Biol Chem. 279: 14879-88. PMID: 14747478
2. Frizzled6 controls hair patterning in mice.  |  Guo, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 9277-81. PMID: 15169958
3. Wnt-4 activates the canonical beta-catenin-mediated Wnt pathway and binds Frizzled-6 CRD: functional implications of Wnt/beta-catenin activity in kidney epithelial cells.  |  Lyons, JP., et al. 2004. Exp Cell Res. 298: 369-87. PMID: 15265686
4. Expression pattern of Wnt signaling components in the adult intestine.  |  Gregorieff, A., et al. 2005. Gastroenterology. 129: 626-38. PMID: 16083717
5. Bone morphogenetic protein-2 modulates Wnt and frizzled expression and enhances the canonical pathway of Wnt signaling in normal keratinocytes.  |  Yang, L., et al. 2006. J Dermatol Sci. 42: 111-9. PMID: 16442268
6. The role of Frizzled3 and Frizzled6 in neural tube closure and in the planar polarity of inner-ear sensory hair cells.  |  Wang, Y., et al. 2006. J Neurosci. 26: 2147-56. PMID: 16495441
7. Mutations in Frizzled 6 cause isolated autosomal-recessive nail dysplasia.  |  Fröjmark, AS., et al. 2011. Am J Hum Genet. 88: 852-860. PMID: 21665003
8. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.  |  Kilander, MB., et al. 2014. Cell Signal. 26: 1943-9. PMID: 24873871
9. Non-canonical Wnt induces chondrocyte de-differentiation through Frizzled 6 and DVL-2/B-raf/CaMKIIα/syndecan 4 axis.  |  Xie, Z., et al. 2018. Cell Death Differ. 25: 1442-1456. PMID: 29352270
10. Molecular mechanisms that regulate export of the planar cell-polarity protein Frizzled-6 out of the endoplasmic reticulum.  |  Tang, X., et al. 2020. J Biol Chem. 295: 8972-8987. PMID: 32376691
11. Molecular cloning of human Frizzled-6.  |  Tokuhara, M., et al. 1998. Biochem Biophys Res Commun. 243: 622-7. PMID: 9480858
12. Identification and characterization of a novel line of Drosophila Schneider S2 cells that respond to wingless signaling.  |  Yanagawa, S., et al. 1998. J Biol Chem. 273: 32353-9. PMID: 9822716

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-frizzled-6 Antibody (C-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-frizzled-6 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

frizzled-6 Antibody (C-12) Specifications

Alternate Product Name: frizzled-6

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 57-79

Epitope Target: frizzled-6

Epitope Species: human

Product Reactivity/Detection: frizzled-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for frizzled-6 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin