Anticorpo EphB2 (2D12C6) raccomandato per il rilevamento di EphB2 di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo EphB2 (2D12C6): sc-130068

Anticorpo EphB2 (2D12C6) valutazione 3.8 di 5 di 6.
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Schede tecniche

    • EphB2 Antibody 2D12C6 è un monoclonale di topo IgG2b κ, citato in 5 pubblicazioni, fornito in 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 17-200 of EphB2 of human origin
    • EphB2 Antibody (2D12C6) é raccomandato per il rilevamento di EphB2 di origine mouse, rat e human in WB, IP, IF e ELISA
    • EphB2 Antibody (2D12C6) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di EphB2 (2D12C6): sc-130068.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for EphB2 Antibody (2D12C6) for WB applications. This reagent is now offered in a bundle with EphB2 Antibody (2D12C6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

请问这个抗体识别的是EphB2的胞外段吗?我做免疫荧光,想染胞膜,不想胞浆着色

Richiesta da: yangli33818
Thank you for your inquiry. EphB2 Antibody (2D12C6): sc-130068 is raised against a recombinant protein corresponding to amino acids 17-200 of EphB2 of human origin. Amino acids 19 – 543 is located at extracellular.And we can provide FREE 10 µg sample of EphB2 (2D12C6): sc-130068. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。EphB2 (2D12C6): sc-130068抗原是人重组EphB2蛋白的17-200氨基酸序列。该蛋白的19-543位于胞外域。另外我们提供10µg 的试用装,可以联系我们亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的中文支持。
Risposta da: Technical Support 9
Data di pubblicazione: 2019-12-27

Are there any issues with using sc-130068: EphB2 (2D12C6) mouse monoclonal primary antibody on mouse samples?

Richiesta da: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-130068 conjugates.
Risposta da: Technical Support
Data di pubblicazione: 2019-12-25
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Rated 1 di 5 di da NO BANDS I tried this antibody in human prostate cancer metastatic cell line LNCaP even at 1:300 dilution but I did not detect any bands.
Data di pubblicazione: 2020-11-04
Rated 5 di 5 di da Good staining by IF Shows strong staining in A549 lung cancer cells by immunofluorescence.
Data di pubblicazione: 2019-06-26
Rated 4 di 5 di da Nice result Worked quite well. Western blot (1:300 dillution).
Data di pubblicazione: 2017-10-31
Rated 5 di 5 di da Great immunofluorescence Used the EphB2 antibody for some Immunofluorescence experiments. Very please with results -- robust labeling and expected receptor localization
Data di pubblicazione: 2017-08-16
Rated 5 di 5 di da EphB2 in brain rats I tried the monoclonal antibody for EphB2 (sc130068). The antibody worked in rats. It is very specific to identify the difference cleavages of this protein in difference parts of neuron.
Data di pubblicazione: 2017-02-16
Rated 3 di 5 di da Positive detection of the truncated human Positive detection of the truncated human recombinant EphB6 protein and of over-expressed EphB6 protein in transfected CHO-K1 cells. -SCBT QC
Data di pubblicazione: 2014-06-06
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