DNA pol α Antibody (G-12): sc-365039

DNA pol α Antibody (G-12) is a mouse monoclonal IgG2a antibody that detects DNA pol α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA pol α plays a crucial role in DNA replication, particularly in the synthesis of the RNA-DNA primer necessary for initiating DNA strand synthesis, which is vital for maintaining genomic integrity. Anti-DNA pol α antibody (G-12) is part of a larger family of DNA polymerases that work collaboratively to ensure accurate DNA replication and repair, thereby preventing mutations that could lead to diseases such as cancer. DNA pol α is essential for proper functioning of the replication machinery, as DNA pol α lays the groundwork for the subsequent action of other polymerases, such as DNA pol δ and DNA pol ε, which take over the elongation of the new DNA strand. DNA pol α monoclonal antibody (G-12) enables researchers to gain insights into the dynamics of DNA replication and the potential implications of dysregulation in various pathological conditions.

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DNA pol α Antibody (G-12) References:

1. Replicative enzymes and ageing: importance of DNA polymerase alpha function to the events of cellular ageing.  |  Srivastava, VK. and Busbee, DL. 2002. Ageing Res Rev. 1: 443-63. PMID: 12067597
2. Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p.  |  Fien, K., et al. 2004. J Biol Chem. 279: 16144-53. PMID: 14766746
3. Calmodulin regulates DNA polymerase alpha activity during proliferative activation of NRK cells.  |  López-Girona, A., et al. 1992. Biochem Biophys Res Commun. 184: 1517-23. PMID: 1590809
4. The mechanism of action of an accessory protein for DNA polymerase alpha/primase.  |  Goulian, M. and Heard, CJ. 1990. J Biol Chem. 265: 13231-9. PMID: 2376593
5. DNA polymerase-α regulates the activation of type I interferons through cytosolic RNA:DNA synthesis.  |  Starokadomskyy, P., et al. 2016. Nat Immunol. 17: 495-504. PMID: 27019227
6. Structural and functional insight into mismatch extension by human DNA polymerase α.  |  Baranovskiy, AG., et al. 2022. Proc Natl Acad Sci U S A. 119: e2111744119. PMID: 35467978
7. Mammalian DNA polymerase alpha holoenzymes with possible functions at the leading and lagging strand of the replication fork.  |  Ottiger, HP. and Hübscher, U. 1984. Proc Natl Acad Sci U S A. 81: 3993-7. PMID: 6588375
8. Accessory proteins for DNA polymerase alpha activity with single-strand DNA templates.  |  Lamothe, P., et al. 1981. Proc Natl Acad Sci U S A. 78: 4723-7. PMID: 6946421
9. DNA polymerase alpha overcomes an error-prone pause site in the presence of replication protein-A.  |  Suzuki, M., et al. 1994. J Biol Chem. 269: 10225-8. PMID: 8144602
10. DNA helicase associated with DNA polymerase alpha: isolation by a modified immunoaffinity chromatography.  |  Biswas, EE., et al. 1993. Biochemistry. 32: 13393-8. PMID: 8257676

The preferred DNA pol α antibody is DNA pol α Antibody (D-7): sc-137021.