Dmc1 Antibody (5B10/2): sc-53270
- Dmc1 Antibody (5B10/2) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against recombinant human Dmc1
- recommended for detection of Dmc1 and Dmc1-D of human origin by WB and IP
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dmc1 Antibody (5B10/2) for WB applications. These reagents are now offered in bundles with Dmc1 Antibody (5B10/2) (see ordering information below).
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Dmc1 Antibody (5B10/2) is a mouse monoclonal IgG2a antibody that detects Dmc1 in human samples through western blotting (WB) and immunoprecipitation (IP) applications. Dmc1 plays a crucial role in meiotic recombination, a process essential for accurate chromosome segregation during gamete formation. Dmc1 is specifically localized to the nucleus, where Dmc1 facilitates DNA double-strand break repair by promoting homologous pairing and strand exchange. Nuclear localization ensures Dmc1 interacts with other key components of the recombination machinery, such as Rad51 and Rad52, which are vital for maintaining genomic stability. Post-translational modifications of Dmc1 can influence activity and interactions, impacting recombination process efficiency. Anti-Dmc1 antibody (5B10/2) serves as an invaluable tool for researchers studying genetic recombination mechanisms and underlying causes of fertility issues and chromosomal disorders in humans.
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Dmc1 Antibody (5B10/2) References:
- Functional interactions of meiotic recombination factors Rdh54 and Dmc1. | Chi, P., et al. 2009. DNA Repair (Amst). 8: 279-84. PMID: 19028606
- The resistance of DMC1 D-loops to dissociation may account for the DMC1 requirement in meiosis. | Bugreev, DV., et al. 2011. Nat Struct Mol Biol. 18: 56-60. PMID: 21151113
- Visualization of human Dmc1 presynaptic filaments. | Sehorn, MG. and Sehorn, HA. 2011. Methods Mol Biol. 745: 485-96. PMID: 21660712
- Tolerance of DNA Mismatches in Dmc1 Recombinase-mediated DNA Strand Exchange. | Borgogno, MV., et al. 2016. J Biol Chem. 291: 4928-38. PMID: 26709229
- Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments. | Crickard, JB., et al. 2018. J Biol Chem. 293: 4191-4200. PMID: 29382724
- High-efficiency genome editing using a dmc1 promoter-controlled CRISPR/Cas9 system in maize. | Feng, C., et al. 2018. Plant Biotechnol J. 16: 1848-1857. PMID: 29569825
- Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase. | Crickard, JB., et al. 2018. Proc Natl Acad Sci U S A. 115: E10041-E10048. PMID: 30301803
- Two auxiliary factors promote Dmc1-driven DNA strand exchange via stepwise mechanisms. | Tsubouchi, H., et al. 2020. Proc Natl Acad Sci U S A. 117: 12062-12070. PMID: 32414915
- RAD51 supports DMC1 by inhibiting the SMC5/6 complex during meiosis. | Chen, H., et al. 2021. Plant Cell. 33: 2869-2882. PMID: 34009315
- RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis. | Bishop, DK. 1994. Cell. 79: 1081-92. PMID: 7528104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dmc1 Antibody (5B10/2) | sc-53270 | 200 µg/ml | $322.00 | |||
Dmc1 Antibody (5B10/2): m-IgG Fc BP-HRP Bundle | sc-536924 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dmc1 Antibody (5B10/2): m-IgGκ BP-HRP Bundle | sc-534099 | 200 µg Ab; 40 µg BP | $361.00 | |||
Dmc1 Antibody (5B10/2): m-IgG2a BP-HRP Bundle | sc-547900 | 200 µg Ab; 10 µg BP | $361.00 |