Anticorpo DGK-alpha (C-11) raccomandato per il rilevamento di DGK-α di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo DGK-α (C-11): sc-271644

Anticorpo DGK-α (C-11) valutazione 4.8 di 5 di 4.
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Schede tecniche

    • Anti-DGK-alpha Antibody C-11 è un monoclonale di topo IgG1 κ DGK-α antibody, citato in 3 pubblicazioni, fornito in 200 µg/ml
    • raised against amino acids 242-298 mapping within an internal region of DGK-α of human origin
    • DGK-alpha Antibody (C-11) é raccomandato per il rilevamento di DGK-α di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • DGK-alpha Antibody (C-11) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di DGK-α (C-11): sc-271644.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DGK-α Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with DGK-α Antibody (C-11) (see ordering information below).
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I have ordered DGK-α Antibody (C-11) Alexa Fluor® 488, sc-271644 AF488 from you and want to try a flow cytometry with this antibody. I wonder do you have the concentration for this antibody and which staining I should use: intracellular or surface?

Richiesta da: Janny
Thank you for your question. sc-271644 AF488 is provided at a concentration of 200 µg in 1 mL. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. Based on IF and IHC(P) staining using the antibody, we observed both cytoplasmic and nuclear staining. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Risposta da: Technical Support
Data di pubblicazione: 2020-02-26

I am using DGK-α (C-11): sc-271644 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Richiesta da: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-03-01
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Rated 4 di 5 di da Great antibody for WB Fine signal and clean background from this antibody!
Data di pubblicazione: 2017-05-16
Rated 5 di 5 di da Highly recommended Highly recommended mouse monoclonal. Strong and specific signal produced by Western blot, IHC and IF
Data di pubblicazione: 2017-01-27
Rated 5 di 5 di da Robust band observed in WB in Jurkat whole Robust band observed in WB in Jurkat whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-06-18
Rated 5 di 5 di da Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Data di pubblicazione: 2015-02-26
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