Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DENND4B Double Nickase Plasmid (h): sc-411384-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DENND4B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DENND4B Double Nickase Plasmid (h) and DENND4B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DENND4B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DENND4B Antibody (F-10): sc-514660
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DENND4B Double Nickase Plasmid (h) | sc-411384-NIC | 20 µg | $410.00 |
DENND4B encodes a DENN domain–containing guanine nucleotide exchange factor that regulates Rab-family small GTPases, supporting membrane trafficking and endosomal recycling. Through modulation of Rab activation states, DENND4B contributes to vesicle tethering/fusion dynamics that influence receptor turnover, nutrient transporter localization, and signaling output from endomembrane compartments. These trafficking functions intersect with pathways controlling cytoskeletal organization, cell polarity, and stress-adaptive responses, making DENND4B a relevant node in studies of cell migration and signal attenuation. Altered regulation of endosomal transport programs linked to DENND4B has been investigated in disease contexts where membrane trafficking and receptor signaling are dysregulated, including cancer-associated cellular remodeling.
DENND4B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DENND4B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DENND4B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DENND4B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DENND4B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.