Anticorpo cyclin B1 (0.N.238) raccomandato per il rilevamento di cyclin B1 di origine mouse, rat e human in WB, IP, IF, IHC(P) e KA

Anticorpo cyclin B1 (0.N.238): sc-70898

Anticorpo cyclin B1 (0.N.238) valutazione 4.5 di 5 di 2.
  • y_2020, m_11, d_29, h_15
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Schede tecniche

    • cyclin B1 Antibody 0.N.238 è un monoclonale di topo IgG1 fornito in 200 µg/ml
    • raised against a recombinant protein corresponding to human cyclin B1
    • raccomandato per il rilevamento di cyclin B1 di origine mouse, rat e human in WB, IP, IF, IHC(P) e KA
    • Vedere cyclin B1 (GNS1): sc-245 for cyclin B1 per anticorpi coniugati, tra cui AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 e 790.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for cyclin B1 Antibody (0.N.238) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin B1 Antibody (0.N.238) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using cyclin B1 (0.N.238): sc-70898?

Richiesta da: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support
Data di pubblicazione: 2017-03-01
  • y_2020, m_11, d_29, h_15CST
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Rated 4 di 5 di da Band at expected size with transfected lysate Band at expected size with transfected lysate and K-562 nuclear extract -SCBT QC
Data di pubblicazione: 2015-01-30
Rated 5 di 5 di da Published data in IF application with U2OS Published data in IF application with U2OS cell lines with and without the constitutive expression of Epstein-Barr virus (EBV) EBNA1 protein and polyomavirus large T antigen -SCBT Publication Review
Data di pubblicazione: 2013-01-22
  • y_2020, m_11, d_29, h_15
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