CD33 Antibody (B-3): sc-514119
- CD33 Antibody (B-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 18-107 mapping within an extracellular domain of CD33 of mouse origin
- Anti-CD33 Antibody (B-3) is recommended for detection of precursor and mature forms of CD33 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CD33 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD33 Antibody (B-3) for WB applications. These reagents are now offered in bundles with CD33 Antibody (B-3) (see ordering information below).
QUICK LINKS
SEE ALSO...
CD33 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD33 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CD33 antibody (B-3) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD33 is a type I transmembrane glycoprotein primarily located on granulocyte and macrophage precursors in bone marrow, while remaining absent from pluripotent stem cells, playing a crucial role in hematopoiesis. CD33 expression on peripheral blood monocytes serves as a significant marker for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. As a sialic acid-dependent cell adhesion molecule, CD33 mediates cell-cell interactions and influences immune responses, making CD33 an important target for therapeutic interventions in hematological malignancies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD33 Antibody (B-3) References:
- Acute myeloid leukemia stem cells and CD33-targeted immunotherapy. | Walter, RB., et al. 2012. Blood. 119: 6198-208. PMID: 22286199
- The effect of CD33 expression on inflammatory response in chronic obstructive pulmonary disease. | Zhang, LX., et al. 2013. Immunol Invest. 42: 701-10. PMID: 23845152
- Gemtuzumab ozogamicin for treatment of newly diagnosed CD33-positive acute myeloid leukemia. | Gbadamosi, M., et al. 2018. Future Oncol. 14: 3199-3213. PMID: 30039981
- CD33+ Immature Myeloid Cells Critically Predict Recurrence in Advanced Gastric Cancer. | Soeno, T., et al. 2020. J Surg Res. 245: 552-563. PMID: 31472311
- Deletion of Alzheimer's disease-associated CD33 results in an inflammatory human microglia phenotype. | Wißfeld, J., et al. 2021. Glia. 69: 1393-1412. PMID: 33539598
- Targeting the membrane-proximal C2-set domain of CD33 for improved CD33-directed immunotherapy. | Godwin, CD., et al. 2021. Leukemia. 35: 2496-2507. PMID: 33589747
- Increasing phagocytosis of microglia by targeting CD33 with liposomes displaying glycan ligands. | Bhattacherjee, A., et al. 2021. J Control Release. 338: 680-693. PMID: 34517042
- A CD33 frameshift variant is associated with neuromyelitis optica spectrum disorders. | Huang, YJ., et al. 2021. Biomed J. 44: S93-S100. PMID: 35735085
- Electrostatic anti-CD33-antibody-protamine nanocarriers as platform for a targeted treatment of acute myeloid leukemia. | Bäumer, N., et al. 2022. J Hematol Oncol. 15: 171. PMID: 36457063
- Plasma proteomic profiling identifies CD33 as a marker of HIV control in natural infection and after therapeutic vaccination. | Duran-Castells, C., et al. 2023. EBioMedicine. 95: 104732. PMID: 37506557
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD33 Antibody (B-3) | sc-514119 | 200 µg/ml | $322.00 | |||
CD33 Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-527855 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD33 Antibody (B-3): m-IgG1 BP-HRP Bundle | sc-533228 | 200 µg Ab; 20 µg BP | $361.00 | |||
CD33 Antibody (B-3) AC | sc-514119 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD33 Antibody (B-3) HRP | sc-514119 HRP | 200 µg/ml | $322.00 | |||
CD33 Antibody (B-3) FITC | sc-514119 FITC | 200 µg/ml | $336.00 | |||
CD33 Antibody (B-3) PE | sc-514119 PE | 200 µg/ml | $349.00 | |||
CD33 Antibody (B-3) Alexa Fluor® 488 | sc-514119 AF488 | 200 µg/ml | $364.00 | |||
CD33 Antibody (B-3) Alexa Fluor® 546 | sc-514119 AF546 | 200 µg/ml | $364.00 | |||
CD33 Antibody (B-3) Alexa Fluor® 594 | sc-514119 AF594 | 200 µg/ml | $364.00 | |||
CD33 Antibody (B-3) Alexa Fluor® 647 | sc-514119 AF647 | 200 µg/ml | $364.00 | |||
CD33 Antibody (B-3) Alexa Fluor® 680 | sc-514119 AF680 | 200 µg/ml | $364.00 | |||
CD33 Antibody (B-3) Alexa Fluor® 790 | sc-514119 AF790 | 200 µg/ml | $364.00 |