BBS2 Double Nickase Plasmid (h): sc-404725-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BBS2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BBS2 Double Nickase Plasmid (h) and BBS2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BBS2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BBS2 Antibody (A-12): sc-365355
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BBS2 Double Nickase Plasmid (h) | sc-404725-NIC | 20 µg | $410.00 | |||
BBS2 Double Nickase Plasmid (h2) | sc-404725-NIC-2 | 20 µg | $410.00 |
BBS2 encodes a core component of the BBSome, a multi-protein complex required for primary cilium assembly and for selective trafficking of membrane receptors and signaling proteins into and out of the cilium. Through its role in ciliary transport, BBS2 influences key cilia-dependent pathways, including Hedgehog and other sensory signaling cascades that coordinate cellular homeostasis and tissue development. Disruption of BBS2 function is linked to Bardet–Biedl syndrome and related ciliopathy phenotypes, making it a widely used target for studying mechanisms of ciliary dysfunction. In human cell models, BBS2 perturbation enables interrogation of receptor localization, vesicular transport dynamics, and downstream transcriptional responses associated with altered ciliary signaling.
BBS2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BBS2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BBS2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BBS2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BBS2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.