ZAP-70 Antibody (A-1): sc-365490

ZAP-70 Antibody (A-1) is a mouse monoclonal IgG2b antibody that detects ZAP-70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZAP-70, a crucial tyrosine kinase, plays a significant role in T cell receptor signaling, which is essential for T lymphocyte activation and subsequent immune responses. Located primarily in the cytoplasm, ZAP-70 associates with the ζ chain of the T cell receptor complex, where ZAP-70 becomes activated through phosphorylation in response to antigen engagement. This activation is vital for propagating downstream signaling pathways that lead to T cell proliferation and differentiation, making ZAP-70 a key player in adaptive immunity. The importance of ZAP-70 is underscored by ZAP-70′s involvement in various immunological disorders; mutations in the ZAP-70 gene can lead to severe combined immunodeficiency, highlighting ZAP-70′s critical function in maintaining a robust immune system. Anti-ZAP-70 antibody (A-1) is therefore an invaluable tool for researchers studying T cell signaling and its implications in health and disease.

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ZAP-70 Antibody (A-1) References:

1. The inhibitory effect of anti-CD33 monoclonal antibodies on AML cell growth correlates with Syk and/or ZAP-70 expression.  |  Balaian, L., et al. 2003. Exp Hematol. 31: 363-71. PMID: 12763134
2. ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain.  |  Chan, AC., et al. 1992. Cell. 71: 649-62. PMID: 1423621
3. Zeta-associated protein of 70 kDa (ZAP-70), but not Syk, tyrosine kinase can mediate apoptosis of T cells through the Fas/Fas ligand, caspase-8 and caspase-3 pathways.  |  Zhong, L., et al. 2004. J Immunol. 172: 1472-82. PMID: 14734724
4. B lymphocytes in humans express ZAP-70 when activated in vivo.  |  Cutrona, G., et al. 2006. Eur J Immunol. 36: 558-69. PMID: 16482508
5. A discrete affinity-driven elevation of ZAP-70 kinase activity initiates negative selection.  |  Mallaun, M., et al. 2010. J Recept Signal Transduct Res. 30: 430-43. PMID: 20945976
6. Prevalence of ZAP-70 and CD 38 in Indian chronic lymphocytic leukemia patients.  |  Gogia, A., et al. 2013. Indian J Cancer. 50: 333-6. PMID: 24369212
7. Validation of ZAP-70 methylation and its relative significance in predicting outcome in chronic lymphocytic leukemia.  |  Claus, R., et al. 2014. Blood. 124: 42-8. PMID: 24868078
8. A Story of Kinases and Adaptors: The Role of Lck, ZAP-70 and LAT in Switch Panel Governing T-Cell Development and Activation.  |  Fernández-Aguilar, LM., et al. 2023. Biology (Basel). 12: PMID: 37759563
9. ZAP-70 and p72syk are signaling response elements through MHC class II molecules.  |  Kanner, SB., et al. 1995. Tissue Antigens. 46: 145-54. PMID: 8525473
10. Increased Zap-70 association with CD3zeta in CD4 T cells from old mice.  |  Garcia, GG. and Miller, RA. 1998. Cell Immunol. 190: 91-100. PMID: 9878110

The preferred ZAP-70 antibody is ZAP-70 Antibody (1E7.2): sc-32760.