Date published: 2025-9-8

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Anticorps V-ATPase D1 (34-Z): sc-81887

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Fiches techniques
  • L'Anticorps V-ATPase D1 (34-Z) est un monoclonal de souris IgG1 κ, cité dans 7 publications, fourni en 100 µg/ml
  • dirigé contre la V-ATPase D1 recombinante d'origine human
  • recommandé pour la détection de V-ATPase D1 d'origine human par WB, IP, IF, IHC(P) et ELISA
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire V-ATPase D1 (34-Z). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for the acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, comprise the V-ATPase complex. Nine subunits (A-H) make up the V1 domain and five subunits (A, D, C, C' and C") make up the V0 domain. V-ATPase D1 (ATPase, H+ transporting, lysosomal, V0 subunit D1), also known as ATP6V0D1, P39, VATX, VMA6, ATP6D or VPATPD, is the D subunit of the V0 domain. Expressed ubiquitously, V-ATPase D1 acts in concert with other V0 subunits to catalytically acidify a variety of intracellular compartments, thereby synthesizing ATP to be used for vacuolar transport.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps V-ATPase D1 (34-Z)

sc-81887
100 µg/ml
$333.00

I detected only 100kDa band and weak band under 37kDa in HUVECs using this antibody. That's why I am asking about the transfected data in the data sheets.  Can this antibody detect other component of V-ATP ase?

Posée par: Maxi
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody for Western blotting.
Répondue par: Tech Service
Date de publication: 2019-11-06

The molecular weight of V-ATPase D1 is 40kDa and the WB data of Hela in the data sheet is 40kDa. But WB of human V-ATPase D1 transfected 293T cells in the data sheet is around 100kDa. Which is the true molecular weight?

Posée par: Maxi
Thank you for your question. Thank you for bringing this discrepancy to our attention. The observed molecular weight in the transfected lysate is much higher than expected. We will be reviewing this data for accuracy and will remove or replace it if necessary.
Répondue par: Technical Support
Date de publication: 2019-11-06

Can sc-81887: V-ATPase D1 (34-Z) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: Professor Griffin
Thank you for your question. Yes, sc-81887: V-ATPase D1 (34-Z) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
  • y_2025, m_8, d_30, h_9CST
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Rated 4 de 5 de par Good specificity observed in WB in HeLa wholeGood specificity observed in WB in HeLa whole cell lysate. -SCBT QC
Date de publication: 2015-04-26
Rated 4 de 5 de par Produced robust immunoperoxidase cytoplasmicProduced robust immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date de publication: 2015-03-31
Rated 4 de 5 de par Published data for WB in HeLa cellsPublished data for WB in HeLa cells. -SCBT Publication Review
Date de publication: 2015-03-28
Rated 4 de 5 de par Good antibody for WB in human VGood antibody for WB in human V-ATPase D1 transfected 293T whole cell lysate. -SCBT QC
Date de publication: 2015-03-13
Rated 4 de 5 de par Published data for WB in epithelial tissuesPublished data for WB in epithelial tissues from human breast cancer tissue. -SCBT Publication Review
Date de publication: 2015-01-12
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Anticorps V-ATPase D1 (34-Z) est évalué 4.0 de 5 de 5.
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