TAP Antibody (D-9): sc-365576

TAP Antibody (D-9) is a mouse monoclonal IgG2b antibody that detects TAP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAP, or tip-associating protein, plays a crucial role in the nuclear export of unspliced viral RNAs, particularly those containing the constitutive transport element (CTE) found in type D retroviruses. By binding directly to the CTE, TAP facilitates the export of these viral RNAs, which is essential for the viral life cycle and the propagation of retroviral infections. TAP is part of a well-characterized mRNA export pathway and belongs to the multigene family of NXF proteins, which are evolutionarily conserved and characterized by a leucine-rich-repeat domain followed by a nuclear transport factor 2-like domain. The structural features of TAP enable direct interaction with various RNA molecules and other cellular components, thereby influencing gene expression and cellular responses to viral infections. Anti-TAP antibody (D-9) serves as an invaluable tool for researchers studying the mechanisms of viral RNA export and the broader implications of mRNA transport in cellular biology.

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TAP Antibody (D-9) References:

1. Epstein-Barr virus protein EB2 contains an N-terminal transferable nuclear export signal that promotes nucleocytoplasmic export by directly binding TAP/NXF1.  |  Juillard, F., et al. 2009. J Virol. 83: 12759-68. PMID: 19793817
2. Evolutionary development of redundant nuclear localization signals in the mRNA export factor NXF1.  |  Zhang, ZC., et al. 2011. Mol Biol Cell. 22: 4657-68. PMID: 21965294
3. The principal mRNA nuclear export factor NXF1:NXT1 forms a symmetric binding platform that facilitates export of retroviral CTE-RNA.  |  Aibara, S., et al. 2015. Nucleic Acids Res. 43: 1883-93. PMID: 25628361
4. An NXF1 mRNA with a retained intron is expressed in hippocampal and neocortical neurons and is translated into a protein that functions as an Nxf1 cofactor.  |  Li, Y., et al. 2016. Mol Biol Cell. 27: 3903-3912. PMID: 27708137
5. The Ebola Virus Nucleoprotein Recruits the Nuclear RNA Export Factor NXF1 into Inclusion Bodies to Facilitate Viral Protein Expression.  |  Wendt, L., et al. 2020. Cells. 9: PMID: 31940815
6. Germline heterozygous mutations in Nxf1 perturb RNA metabolism and trigger thrombocytopenia and lymphopenia in mice.  |  Chappaz, S., et al. 2020. Blood Adv. 4: 1270-1283. PMID: 32236527
7. Structure and function of the porcine TAP protein and its inhibition by the viral immune evasion protein ICP47.  |  Chai, HH., et al. 2021. Int J Biol Macromol. 178: 514-526. PMID: 33662419

The preferred TAP antibody is TAP Antibody (53H8): sc-32319.