Synaptotagmin VII CRISPR Activation Plasmid (h): sc-404291-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptotagmin VII CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Synaptotagmin VII CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Synaptotagmin VII CRISPR Activation Plasmid (h) and Synaptotagmin VII CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the SYT7 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synaptotagmin VII Antibody (4H4): sc-293343
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptotagmin VII CRISPR Activation Plasmid (h) | sc-404291-ACT | 20 µg | $397.00 |
SYT7 encodes synaptotagmin VII, a Ca2+-sensing membrane protein that regulates Ca2+-dependent exocytosis and membrane repair through SNARE-mediated vesicle fusion. It participates in secretory lysosome exocytosis, plasma membrane resealing after injury, and regulated release of cargos in immune and neuronal-like cells, linking calcium signaling to vesicular trafficking. Synaptotagmin VII activity influences processes such as neurotransmitter release dynamics, cytokine secretion, and endosomal/lysosomal trafficking. Altered SYT7 expression or function has been associated with dysregulated secretion and membrane homeostasis phenotypes relevant to neurobiology, inflammation, and cellular stress responses.
Synaptotagmin VII CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous SYT7 expression without altering the underlying DNA sequence.
Synaptotagmin VII CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the SYT7 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the SYT7 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Synaptotagmin VII expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native SYT7 locus and enabling the study of Synaptotagmin VII-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Synaptotagmin VII pathway restoration in tumor cells with silenced or reduced SYT7 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.