Anticorps SREBP-2 (A-12) recommandé pour la détection de SREBP-2 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps SREBP-2 (A-12): sc-271616

Anticorps SREBP-2 (A-12) est évalué 4.3 de 5 de 9.
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Fiches techniques

    • L'Anticorps SREBP-2 (A-12) est un monoclonal de souris IgG2a κ, cité dans 8 publications, fourni en 200 µg/ml
    • dirigé contre les acides aminés 812-975 de SREBP-2 d'origine human
    • Anti-L'Anticorps SREBP-2 (A-12) est recommandé pour la détection de SREBP-2 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Anti-L'Anticorps SREBP-2 (A-12) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de SREBP-2 (A-12): sc-271616.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SREBP-2 Antibody (A-12). This work is in progress.
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Does this antibody recognize also the processed (activated) form of SREBP?

Posée par: LaMa
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Répondue par: Technical Support
Date de publication: 2021-07-22

I am using SREBP-2 (A-12): sc-271616 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Posée par: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 1 de 5 de par no result I used a free sample for SREBP2 detection in WB of protein extracts from H9c2 cells. Unfortunately I did not get any results although I have tested various dilutions of the antibody (1: 1000, 1: 500, 1: 250).
Date de publication: 2019-12-13
Rated 5 de 5 de par expected results SREBP-2 expression in the rat kidney of type I diabetic, and the results of the antibodies were consistent with the expected results. It was recommended that the dilution should be controlled from 1:800 to 1:1000.
Date de publication: 2017-09-16
Rated 5 de 5 de par very sensitive This SREBP-2 antibody is very sensitive. The result gives no background with a very strong signal.I suggest use 1:800 dilution and incubtated at 4℃ overnight instead of RT incubated.
Date de publication: 2017-09-09
Rated 5 de 5 de par recommend use Strong and specific, this clone is a great reliable choice for IHC and WB analysis.
Date de publication: 2017-05-13
Rated 5 de 5 de par IHC staining IHC staining result is strong for mouse liver tissue slide sections, 1:300 been diluted.
Date de publication: 2017-02-12
Rated 5 de 5 de par Well-rounded antibody for SREBP-2 Reliable antibody that gives great staining in IHC on paraffin sections and strong solid bands in Western blot. Excellent choice!
Date de publication: 2017-02-01
Rated 4 de 5 de par A large intense band observed by Western A large intense band observed by Western Blot using MOLT-4 whole cell lysate. -SCBT QC
Date de publication: 2015-05-17
Rated 5 de 5 de par Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date de publication: 2015-05-01
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