Date published: 2025-9-9

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Anticorps Ribosomal Protein S6 (H-4): sc-74576

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Fiches techniques
  • L'Anticorps Ribosomal Protein S6 (H-4) est un monoclonal de souris IgG1 κ, cité dans 10 publications, fourni en 200 µg/ml
  • soulevé contre le N-terminus de Ribosomal Protein S6 d'origine human.
  • recommandé pour la détection de 40S Ribosomal Protein S6 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • m-IgG Fc BP-HRP et m-IgG1 BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps Ribosomal Protein S6 (H-4) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps Ribosomal Protein S6 (H-4) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The genes encoding for mammalian ribosomal proteins comprise multigene families that consist predominantly of multiple processed pseudogenes and one functional intro-containing gene within their coding regions. The rpS6 gene gives rise to Ribosomal protein S6 (also designated RPS6). RPS6 is the major substrate of protein kinases in eukaryotic ribosomes. Sequence comparison has identified RPS6 as the equivalent of the ribosomal protein S10 from Saccharomyces cerevisiae. The sequence comparison of ribosomal proteins from evolutionarily distant eukaryotes, such as yeast and human, indicates that the structure and probably the function of RPS6 has been highly conserved.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Ribosomal Protein S6 (H-4)

sc-74576
200 µg/ml
$316.00

Ribosomal Protein S6 (H-4): m-IgG Fc BP-HRP Kit

sc-539551
200 µg Ab; 10 µg BP
$354.00

Ribosomal Protein S6 (H-4): m-IgG1 BP-HRP Kit

sc-541552
200 µg Ab; 20 µg BP
$354.00

I am using Ribosomal Protein S6 (H-4): sc-74576 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Posée par: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Works with yeast S. cerevisaeThis antibody has good sensitivity and specificity towards yeast S. cerevisae Rps6. Yeast lysates were collected using the culture boiling technique resuspending in 1x loading buffer (30 uL/1.0 OD600*mL cells collected) and loading 5 uL per well (https://doi.org/10.1002/yea.1628). Performed in quadruplicate. Total protein staining by R-Prob Sigma kit to show consistent protein levels per lane. Antibody used at 1:1000 dilution.
Date de publication: 2021-07-28
Rated 5 de 5 de par Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in formalin-fixed Hep G2 cells. -SCBT QC
Date de publication: 2015-04-10
Rated 4 de 5 de par No commentNo comment
Date de publication: 2015-03-25
Rated 5 de 5 de par Very robust signal in WB in mouse RibosomalVery robust signal in WB in mouse Ribosomal Protein S6 transfected 293T and K-562 whole cell lysates. -SCBT QC
Date de publication: 2015-03-21
Rated 5 de 5 de par Robust band seen in WB in Hep G2 and NIH/3T3Robust band seen in WB in Hep G2 and NIH/3T3 whole cell lysates. -SCBT QC
Date de publication: 2015-03-10
Rated 5 de 5 de par Very strong band seen in WB in HeLaVery strong band seen in WB in HeLa, K-562 and KNRK whole cell lysates. -SCBT QC
Date de publication: 2015-03-06
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Anticorps Ribosomal Protein S6 (H-4) est évalué 4.8 de 5 de 6.
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