Anticorps Ribosomal Protein S6 (C-8) recommandé pour la détection de 40S Ribosomal Protein S6 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps Ribosomal Protein S6 (C-8): sc-74459

Anticorps Ribosomal Protein S6 (C-8) est évalué 4.8 de 5 de 29.
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Fiches techniques

    • Anti-L'anticorps Ribosomal Protein S6 C-8 est un monoclonal IgG2b (chaîne légère kappa) L'Anticorps Ribosomal Protein S6 fourni en 200 µg/ml
    • raised against amino acids 1-249 mapping at the N-terminus of Ribosomal Protein S6 of human origin
    • Anti-L'Anticorps Ribosomal Protein S6 (C-8) est recommandé pour la détection de 40S Ribosomal Protein S6 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Anti-L'Anticorps Ribosomal Protein S6 (C-8) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Ribosomal Protein S6 (C-8): sc-74459.
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is sc-74459 tested in plant?this is my sequence:MKFNIANPTTGCQKKLEIDDDQKLRAFFDKRISQEVSGDALGDEFKGYVFKIMGGCDKQGFPMKQGVLTPGRVRLLLHRGTPCFRGYGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEKDLPGLTDTEKPRMRGPKRASKIRKLFNLSKEDDVRKYVNTYRRNFTTKTGKTASKAPKIQRLVTPLTLQRKRARIADKKKRIAKA

Posée par: fakih
Thank you for your question. This antibody has not been tested on plant samples, and based on sequence homology we can not recommend this antibody for plant use.
Répondue par: Tech Service
Date de publication: 2019-04-29

Hi Has this conjugated FITC antibody ever been tested in Flow cytometry? Cheers

Posée par: SJT2
We have not yet tested the FITC conjugated antibody in FCM. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Répondue par: Technical Support
Date de publication: 2019-04-13

Is there any citation using this antibody for IP? Would be easier if you could filter publications accordingly with the technique needed. 

Posée par: Ioris
Thanks for your question. You can find the pubblications related to the desired application and much more following this link: https://www.scbt.com/scbt/misc/citationSearch.jsp . Please contact our Technical Service if you need any further support.
Répondue par: SCBT Heidelberg Support
Date de publication: 2019-01-06

Does this antibody work equally in PBS and TBS?

Posée par: Muriel74
Thank you for your question. Yes, this product is provided in PBS, but will also work if diluted in TBS. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Répondue par: Tech Service
Date de publication: 2017-01-12

Are there any issues with using this mouse monoclonal primary antibody on mouse samples?

Posée par: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, our Ribosomal Protein S6 sc-74459 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Répondue par: Technical Support 12
Date de publication: 2017-01-04
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Rated 5 de 5 de par Excellent antibody I used this sample to detect S6 protein on A549 and CL1-5 cells and had great bands intensity and specific binding.
Date de publication: 2020-05-10
Rated 4 de 5 de par IOutstanding antiibody! We used this antibody to selectively detect total ribosomal protein S6 in PC-3 cells by Western blot. When used at 1:10,000 (in TBST-3% BSA) this antibody yields a single band at the expected MW. Inhibiting mTOR with rapamycin or YM155, as expected, does not inhibit this signal.
Date de publication: 2020-04-12
Rated 5 de 5 de par s6 ribosomal protein SINGLE BAND DETECTED WITH THE EXPECTED MOLECULAR MASS
Date de publication: 2019-09-12
Rated 3 de 5 de par 適切なサイズで検出できました。 無料サンプルRPS6(c-8)抗体いただきましたので、MEF細胞でwestern blotによる チェックを行いました。適切なサイズでバンドが検出できました。
Date de publication: 2019-05-20
Rated 5 de 5 de par Fine for WB We tried 1:1000 with neuroblastoma cells and worked nicely.
Date de publication: 2018-11-01
Rated 5 de 5 de par clean strong signal Detected a single clean band of RPS6 from human glial cells (50 µg protein/lane) in a Western blot using a 1:200 dilution of primary antibody, 1:10,000 dilution of secondary antibody, and Super Signal Pico chemiluminescence.
Date de publication: 2018-02-20
Rated 5 de 5 de par Impossibile trovare di meglio! Ho testato questo Ab in Immunofluorescenza otteneno immagini eccezionali. Lo consiglio, è ottimo.
Date de publication: 2017-11-28
Rated 5 de 5 de par great antibody I have used this antibody at 1:500 dilution on murine spelnic B cell extracts and it worked beautifully. Highly recommended.
Date de publication: 2017-11-14
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