Anticorps Ribosomal Protein L11 (2A1) recommandé pour la détection de Ribosomal Protein L11 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps Ribosomal Protein L11 (2A1): sc-293224

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Fiches techniques

    • Anti-L'anticorps Ribosomal Protein L11 2A1 est un monoclonal IgG2b (chaîne légère kappa) L'Anticorps Ribosomal Protein L11 fourni en 100 µg/ml
    • dirigé contre les acides aminés 1-177 correspondant à la séquence totale de Ribosomal Protein L11 d'origine human
    • recommandé pour la détection de Ribosomal Protein L11 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.

Why is your antibody recognising a 40 kDa protein - RPL11 is about 20 kDa. It should also be predominantly cytoplasmic - as that is where the ribosomes are. My lab works on ribosome biogenesis and I think this antibody should be removed from your stock

Posée par: NickWatkins
Thank you very much for the question! The full-length Ribosomal Protein L11 of human origin has 177 amino acids. The calculated molecular weight of this protein to be detected by standard WB techniques is expected to be around 20kDa. This is also the weight we refer to on the corresponding product datasheet you can download by clicking the following link: https://datasheets.scbt.com/sc-293224.pdf The WB image we show on this product page is showing a Ribosomal Protein L11 - GST fusion protein with the corresponding higher molecular weight. This is the correct mw size we expected in this control experiment but of course not the size to be detected on endogenously expressed Ribosomal Protein L11. In the IF staining of methanol-fixed HeLa cells our labs have performed this antibody was showing nuclear localization. According to the protein literature we found this protein should be expressed predominantly in the cytoplasma but we also found supporting information about IF localization in the nucleus. Our labs will look further into this and post additional data here on the product page upon availability.
Répondue par: Technical Support Europe
Date de publication: 2017-09-18

Why is the RPL11 signal in the nucleus when RPL11 is predominantly a cytoplasmic protein? This suggests to me that the antibody is non-functional

Posée par: NickWatkins
Thank you for your question. HPRD and UniProt list nuclear and nucleolar as subcellular localizations for RP11; links to their websites are provided below for reference. However, we will also ask our lab to reevaluate the data based on your feedback. If we are able to provide any new or revised data, it will be updated on our website. http://www.uniprot.org/uniprot/P62913#subcellular_location http://www.hprd.org/summary?hprd_id=10364&isoform_id=10364_1&isoform_name=Isoform_1
Répondue par: Technical Support
Date de publication: 2017-08-01

Can sc-293224: Ribosomal Protein L11 (2A1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: Professor Griffin
Thank you for your question. Yes, sc-293224: Ribosomal Protein L11 (2A1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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