Rad GTPase Antibody (B-10): sc-373988
- Rad GTPase Antibody (B-10) is a mouse monoclonal IgG2a κ Rad GTPase antibody provided at 200 µg/ml
- raised against amino acids 1-143 mapping at the N-terminus of Rad GTPase of human origin
- Rad GTPase Antibody (B-10) is recommended for detection of Rad GTPase of human origin by WB, IP, IF and ELISA
- Anti-Rad GTPase Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rad GTPase Antibody (B-10) for WB applications. These reagents are now offered in bundles with Rad GTPase Antibody (B-10) (see ordering information below).
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SEE ALSO...
Rad GTPase Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rad GTPase of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad GTPase (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad GTPase plays a crucial role in cellular signaling pathways, particularly in regulating vascular smooth muscle cell proliferation and migration, which are essential processes in maintaining vascular health and preventing diseases such as atherosclerosis. Unlike other members of the Ras family, Rad GTPase is unique in lacking typical prenylation motifs at its C terminus, which affects localization and function within cells. Rad GTPase is primarily expressed in skeletal muscle, cardiac muscle, and lung tissues, and overexpression in skeletal muscle has been linked to type II diabetes, highlighting potential involvement in metabolic disorders. Understanding Rad GTPase function and regulation is vital for developing therapeutic strategies targeting vascular diseases and metabolic conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rad GTPase Antibody (B-10) References:
- Interaction of the Ras-related protein associated with diabetes rad and the putative tumor metastasis suppressor NM23 provides a novel mechanism of GTPase regulation. | Zhu, J., et al. 1999. Proc Natl Acad Sci U S A. 96: 14911-8. PMID: 10611312
- Rad GTPase attenuates vascular lesion formation by inhibition of vascular smooth muscle cell migration. | Fu, M., et al. 2005. Circulation. 111: 1071-7. PMID: 15710763
- Opposing roles for DNA structure-specific proteins Rad1, Msh2, Msh3, and Sgs1 in yeast gene targeting. | Langston, LD. and Symington, LS. 2005. EMBO J. 24: 2214-23. PMID: 15920474
- Inverted repeat-stimulated sister-chromatid exchange events are RAD1-independent but reduced in a msh2 mutant. | Nag, DK., et al. 2005. Nucleic Acids Res. 33: 5243-9. PMID: 16166656
- Characterization of Rad, a new member of Ras/GTPase superfamily, and its regulation by a unique GTPase-activating protein (GAP)-like activity. | Zhu, J., et al. 1995. J Biol Chem. 270: 4805-12. PMID: 7876254
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad GTPase Antibody (B-10) | sc-373988 | 200 µg/ml | $322.00 | |||
Rad GTPase Antibody (B-10): m-IgGκ BP-HRP Bundle | sc-522582 | 200 µg Ab, 40 µg BP | $361.00 | |||
Rad GTPase Antibody (B-10): m-IgG2a BP-HRP Bundle | sc-546118 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rad GTPase Antibody (B-10) AC | sc-373988 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rad GTPase Antibody (B-10) HRP | sc-373988 HRP | 200 µg/ml | $322.00 | |||
Rad GTPase Antibody (B-10) FITC | sc-373988 FITC | 200 µg/ml | $336.00 | |||
Rad GTPase Antibody (B-10) PE | sc-373988 PE | 200 µg/ml | $349.00 | |||
Rad GTPase Antibody (B-10) Alexa Fluor® 488 | sc-373988 AF488 | 200 µg/ml | $364.00 | |||
Rad GTPase Antibody (B-10) Alexa Fluor® 546 | sc-373988 AF546 | 200 µg/ml | $364.00 | |||
Rad GTPase Antibody (B-10) Alexa Fluor® 594 | sc-373988 AF594 | 200 µg/ml | $364.00 | |||
Rad GTPase Antibody (B-10) Alexa Fluor® 647 | sc-373988 AF647 | 200 µg/ml | $364.00 | |||
Rad GTPase Antibody (B-10) Alexa Fluor® 680 | sc-373988 AF680 | 200 µg/ml | $364.00 | |||
Rad GTPase Antibody (B-10) Alexa Fluor® 790 | sc-373988 AF790 | 200 µg/ml | $364.00 |