Date published: 2025-9-7

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Anticorps PU.1/Spi1 (B-9): sc-390659

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Fiches techniques
  • L'Anticorps PU.1/Spi1 (B-9) est un monoclonal de souris IgG1 κ, cité dans 3 publications, fourni en 200 µg/ml
  • spécifique d'un épitope situé entre les acides aminés 239-271 au niveau C-terminus de PU.1 d'origine mouse
  • recommandé pour la détection de PU.1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • Réactifs TransCruz pour une application en ChIP (sc-390659 X, 200 µg/0.1 ml)
  • Voir PU.1/Spi1 (C-3): sc-390405 pour d'autres anticorps conjugués PU.1/Spi1, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • m-IgG Fc BP-HRP et m-IgG1 BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps PU.1/Spi1 (B-9) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps PU.1/Spi1 (B-9) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T-cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T-lineage commitment and then is dramatically absent following the beta-selection checkpoint.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps PU.1/Spi1 (B-9)

sc-390659
200 µg/ml
$316.00

PU.1/Spi1 (B-9): m-IgG Fc BP-HRP Kit

sc-540830
200 µg Ab; 10 µg BP
$354.00

PU.1/Spi1 (B-9): m-IgG1 BP-HRP Kit

sc-542399
200 µg Ab; 20 µg BP
$354.00

PU.1/Spi1 (B-9) peptide neutralisant

sc-390659 P
100 µg/0.5 ml
$68.00

Anticorps PU.1/Spi1 (B-9) X

sc-390659 X
200 µg/0.1 ml
$316.00

What application is the blocking peptide sc-390659 P appropriate for?

Posée par: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Great monoclonal antibodyNice strong signal by Western blot with little to no background. Strong robust signal in IHCP
Date de publication: 2017-02-06
Rated 5 de 5 de par Satisfactory Western blot data of PUSatisfactory Western blot data of PU.1 expression in K-562 nuclear extract. -SCBT QC
Date de publication: 2013-05-07
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Anticorps PU.1/Spi1 (B-9) est évalué 5.0 de 5 de 2.
  • y_2025, m_8, d_29, h_8
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