Positive cofactor 4 Antibody (H-8): sc-166279

Positive cofactor 4 Antibody (H-8) is a mouse monoclonal IgG2a antibody that detects Positive Cofactor 4 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Positive Cofactor 4 plays a crucial role in the regulation of transcription within eukaryotic cells, acting as a coactivator that mediates the interaction between transcriptional activators and the RNA polymerase II holoenzyme. Positive Cofactor 4 is essential for both basal and gene-specific transcriptional activation, as Positive Cofactor 4 associates with various cofactors, including transcription factor IIA and TATA-binding protein-associated factors, to facilitate the binding of the transcription machinery to double-stranded DNA. The ability of Positive Cofactor 4 to induce transcriptional activation or repression depends on the presence of specific transcription factors and holoenzyme components, highlighting Positive Cofactor 4′s importance in the precise regulation of gene expression. Additionally, Positive Cofactor 4 undergoes phosphorylation by TFIID and TFIIH, a post-translational modification that plays a significant role in function by releasing Positive Cofactor 4 from the DNA promoter region, thereby inhibiting assembly into the transcriptional promoting complex and blocking transcription. This dynamic regulation underscores Positive Cofactor 4′s role in maintaining the balance of gene expression, which is vital for cellular function and response to environmental signals.

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Positive cofactor 4 Antibody (H-8) References:

1. Coactivator function of positive cofactor 4 (PC4) in Sp1-directed luteinizing hormone receptor (LHR) gene transcription.  |  Liao, M., et al. 2011. J Biol Chem. 286: 7681-91. PMID: 21193408
2. Human positive coactivator 4 (PC4) is involved in the progression and prognosis of astrocytoma.  |  Chen, L., et al. 2014. J Neurol Sci. 346: 293-8. PMID: 25262015
3. Positive Cofactor 4 (PC4) is critical for DNA repair pathway re-routing in DT40 cells.  |  Caldwell, RB., et al. 2016. Sci Rep. 6: 28890. PMID: 27374870
4. Positive cofactor 4 (PC4) contributes to the regulation of replication-dependent canonical histone gene expression.  |  Brzek, A., et al. 2018. BMC Mol Biol. 19: 9. PMID: 30053800
5. Transcriptional positive cofactor 4 promotes breast cancer proliferation and metastasis through c-Myc mediated Warburg effect.  |  Luo, P., et al. 2019. Cell Commun Signal. 17: 36. PMID: 30992017
6. The human positive cofactor 4 promotes androgen-independent prostate cancer development and progression through HIF-1α/β-catenin pathway.  |  Luo, P., et al. 2019. Am J Cancer Res. 9: 682-698. PMID: 31105996
7. The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma.  |  Sun, T., et al. 2021. J Oncol. 2021: 9958483. PMID: 34899911
8. Positive Cofactor 4 as a Potential Radiation Biodosimeter for Early Assessment.  |  Ma, L., et al. 2022. Dose Response. 20: 15593258221081317. PMID: 35221823
9. Targeting positive cofactor 4 induces autophagic cell death in MYC-expressing diffuse large B-cell lymphoma.  |  Ma, L., et al. 2023. Exp Hematol. 119-120: 42-57.e4. PMID: 36642374
10. PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair.  |  Pan, Q., et al. 2023. J Biol Chem. 299: 105032. PMID: 37437887

The preferred Positive cofactor 4 antibody is Positive cofactor 4 Antibody (H-12): sc-166280.