Date published: 2025-9-7

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Anticorps Notch1 (A-8): sc-376403

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Fiches techniques
  • L'anticorps Notch1 A-8 est un monoclonal IgM κ L'Anticorps Notch1, cité dans 50 publications, fourni en 200 µg/ml
  • spécifique d'un épitope situé entre les acides aminés 2425-2444 près de C-terminus de Notch 1 d'origine human
  • recommandé pour la détection de Notch 1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; à la phycoérythrine, à la fluorescéine, à l'Alexa Fluor® 488 ou à l'Alexa Fluor® 647 pour IF, IHC(P) et FCM
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Notch1 (A-8): sc-376403.
  • Le m-IgGκ BP-HRP est le réactif de détection secondaire préféré pour l'anticorps Notch1 (A-8) pour les applications WB et IHC(P). Ce réactif est désormais proposé en association avec l'anticorps Notch1 (A-8)(voir les informations relatives à la commande ci-dessous). Pour d'autres conjugués m-IgGκ BP, voir notre liste complète de protéines de liaison à l'IgG de souris.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Notch 1 Antibody (A-8) is an IgM κ mouse monoclonal Notch 1 antibody (also designated NOTCH1 antibody) that detects the Notch 1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Notch 1 Antibody (A-8) is available as both the non-conjugated anti-Notch 1 antibody form, as well as multiple conjugated forms of anti-Notch 1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The LIN-12/Notch transmembrane receptors are believed to play a central role in development by regulating cell fate decisions. Four Notch homologs (Notch 1, Notch 2, Notch 3 and Notch 4) have been identified in mammals. The Notch genes are expressed in a variety of embryonic and adult tissues, suggesting that the genes are involved in multiple signaling pathways. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged1 can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 may be involved in tissue development that is dependent upon epithelial-mesenchymal interactions. In addition to its normal expression in the adrenal gland and placenta, Delta expression has also been found in neuroendocrine tumors.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Notch1 (A-8)

sc-376403
200 µg/ml
$316.00

Notch1 (A-8): m-IgGκ BP-HRP Kit

sc-552043
200 µg Ab; 40 µg BP
$354.00

Anticorps Notch1 (A-8) AC

sc-376403 AC
500 µg/ml, 25% agarose
$416.00

Anticorps Notch1 (A-8) Alexa Fluor® 488

sc-376403 AF488
200 µg/ml
$357.00

Anticorps Notch1 (A-8) Alexa Fluor® 647

sc-376403 AF647
200 µg/ml
$357.00

Anticorps Notch1 (A-8) FITC

sc-376403 FITC
200 µg/ml
$330.00

Anticorps Notch1 (A-8) HRP

sc-376403 HRP
200 µg/ml
$316.00

Notch1 (A-8) peptide neutralisant

sc-376403 P
100 µg/0.5 ml
$68.00

Anticorps Notch1 (A-8) PE

sc-376403 PE
200 µg/ml
$343.00

Hello, What secondary antibody should i use for WB? I can't see anywhere the host species,..Thanks

Posée par: myrto
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Répondue par: Dallas Tech Service
Date de publication: 2021-04-20

Hello, I would like to use Notch-1 Antibody (A-8) primarily for western blot and later maybe for IC. Could you let me know if I could purchase the antibody conjugated to Alexa Fluor 488 and use it for WB Too or for WB only the HRP conjugated works?

Posée par: myrto
Yes, you can use conjugates to Alexa Fluor 488 for Western blotting.
Répondue par: Tech Sales
Date de publication: 2021-03-15

Can I use this antibody for IF-P staining? If not which product can I use for IF tissue staining?

Posée par: JordanA
Thank you for your question. This antibody is recommended for IF and IHC(P), or immunofluorescence and immunohistochemistry on formalin-fixed paraffin-embedded tissue sections.
Répondue par: Technical Support
Date de publication: 2019-07-18

I purchased this Notch 1 antibody (A-8) to do western blot for human breast cancer cell lines lysates, but I am not sure where the band will appear in the gel (110KD or 210 KD?). Please tell me a relatively accurate position. THX~

Posée par: Tianzi
Thank you for your question. The expected molecular weight for Notch one is ~300 kDa (precursor) and two possible cleaved forms which run around ~180 and ~115-120kDa.
Répondue par: Tech Service
Date de publication: 2021-04-19

Hi, I am intersted in Notch 1 Antibody (A-8) Alexa Fluor® 647 (sc-376403 AF647, 314USD) I just saw its concentration is 200ug/mL. How about the volume? Thanks.

Posée par: Bin Zhao
Thank you for your question. It is provided as 1ml.
Répondue par: Tech Service
Date de publication: 2018-11-21

I would like to use Notch 1 Antibody (A-8): sc-376403 for double staining with another mouse monoclonal primary antibody. Is this possible?

Posée par: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376403 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Répondue par: Technical Support 15
Date de publication: 2017-01-26
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Rated 5 de 5 de par Notch1 worked very well in IHCNotch1 worked very well in IHC in human tissues by DAB staining
Date de publication: 2023-05-16
Rated 4 de 5 de par Buon anticorpo per WB su campioni proteici umanitestato per western blot ad una concentrazione di 1:1000 su estratti proteici totali di cellule THP1 e su LX2 umane. tempo di esposizione rapido, banda netta
Date de publication: 2022-11-21
Rated 1 de 5 de par No staining in murine heartThe antibody has been tested with different antigen retrival protocols. None of them worked out.
Date de publication: 2022-03-03
Rated 4 de 5 de par Good for flow cytometryThis antibody works well for flow cytometry at 1:50 but requires permeabilization of the cell membrane.
Date de publication: 2019-02-26
Rated 4 de 5 de par THIS IS A GOOD ANTIBODYI got a free sample of this antibody and it is working very fine. I am interested in buying a new vial.
Date de publication: 2018-03-29
Rated 4 de 5 de par Antibody works greatBought this Notch1 monoclonal A8 Ab. Works better than Notch C-20 antibody. Specificity is good
Date de publication: 2018-02-08
Rated 3 de 5 de par be patient !This antibodies is working just fine but for me I just need to wait and give it a little long exposure time..
Date de publication: 2017-12-21
Rated 2 de 5 de par backgroundthe antibody was tested on rat tissue samples in western blot. A signal is detected but with a high background level
Date de publication: 2017-12-13
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Anticorps Notch1 (A-8) est évalué 4.3 de 5 de 27.
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