Anticorps MMP-2 (2C1) recommandé pour la détection de MMP-2 d'origine mouse, rat et human par WB, IP et IF

Anticorps MMP-2 (2C1): sc-13594

Anticorps MMP-2 (2C1) est évalué 4.3 de 5 de 41.
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Fiches techniques

    • Anti-L'anticorps MMP-2 2C1 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps MMP-2 fourni en 200 µg/ml
    • dirigé contre la forme activée de MMP-2 de human
    • Anti-L'Anticorps MMP-2 (2C1) est recommandé pour la détection de MMP-2 d'origine mouse, rat et human par WB, IP et IF
    • L'Anticorps MMP-2 (2C1) est disponible conjugué à l'agarose pour IP; et soit à la phycoerythrin ou FITC our IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de MMP-2 (2C1): sc-13594.

Does it work for bovine tissue?

Posée par: Anonyme
Thank you for your question. This antibody has not yet been tested in bovine samples and so that is not a recommended species at this time. We are still gathering data to show on our website. We do have a previous citation in bovine samples which may be helpful for you: PMID: 12844104.
Répondue par: Tech Service
Date de publication: 2019-11-20

We are looking for a MMP-2 antibody that works well in Western blotting . For this we would like to use a 10ug free sample of MMP2 antibody (2C1): sc-13594. I was wondering if we could get some.

Posée par: Shiv
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Répondue par: Technical Support
Date de publication: 2019-02-26

Hello.. is there a recommended buffer for use of this product with tissue lysates and immunoblotting? We usually use RIPA buffer for protein expression, but was concerned about chelators.

Posée par: Nabilb
Thank you for your question. We do not have any specific buffer recommendation for use with this MMP-2 antibody and have prepared our lysates for testing using our standard RIPA buffer. You may want to refer to some of the numerous product citations using this antibody for Western blotting to see if they have any optimized recommendations.
Répondue par: Technical Support
Date de publication: 2019-01-26

What is the epitope sequence (binding site) of this antibody? Could you tell me a.a. number?

Posée par: neoSong
Thank you for your question. This mouse monoclonal antibody, MMP-2 (2C1): sc-13594, is raised against the activated form of MMP-2 of human origin. The exact epitope for this monoclonal antibody has not yet been determined.
Répondue par: Technical Support
Date de publication: 2017-09-14

Can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Posée par: Anna99
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This monoclonal for MMP-2 is available conjugated to agarose (sc-13594 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-13594 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-13594 PE), fluorescein (sc-13594 FITC), Alexa Fluor® 488 (sc-13594 AF488) or Alexa Fluor® 647 (sc-13594 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Répondue par: Technical Support 13
Date de publication: 2017-01-04
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Rated 5 de 5 de par UPENN Gα 12 Antibody is very good. The band is very stronger.
Date de publication: 2018-04-19
Rated 3 de 5 de par 抗体特异性还可以 用着效果还行。带的大小有些不确定,最上一条带在70kd以下,认为是剪切的mmp2,所以下面的条带不确定是什么。
Date de publication: 2018-03-20
Rated 5 de 5 de par Great product MMP-2 antibody works well at the concentration of 1:500 with satisfied bands
Date de publication: 2018-02-08
Rated 5 de 5 de par western blot This antibody generates a clearsignal with the HepG2 cell lysis (1:200).
Date de publication: 2018-01-25
Rated 1 de 5 de par Unsucessful western blot with glioblastoma cells I have used this antibody 1:100-1:200 for western blotting and failled to obtain any band with the expected molecular weight using lysates from human glioblastoma cell lines
Date de publication: 2017-11-28
Rated 2 de 5 de par May not be suitable for IF Sample:muscle cryosection Applications: IF 1:100, 1:200, 1:400 Results: Very weak staining. A little bit staining in nuclear. No difference with negetive control.
Date de publication: 2017-11-22
Rated 1 de 5 de par Didnot work for WB did not work for western - samples were human cells in culture
Date de publication: 2017-11-21
Rated 5 de 5 de par work well 一月前我向贵公司申请了该抗体的试用装,结果非常好,条带清楚明显,位置正确,抗体很不错,可靠有用,值得再次购买
Date de publication: 2017-10-19
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