MLK3 Antibody (D-11): sc-166639

MLK3 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects MLK3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLK3 Antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLK3, also known as mixed lineage kinase 3, plays a crucial role in cellular signaling pathways, particularly in stress response regulation and cell differentiation. MLK3 functions as a non-receptor protein kinase spanning 847 amino acids and features a unique structure including an SH3 domain, essential for protein-protein interactions, and a leucine zipper basic region often associated with transcription factors. Proline-rich motifs in MLK3′s long carboxy-terminal tail enable interactions with other signaling proteins, influencing various cellular processes. Such interaction proves vital for signal transduction from growth factor receptors, ultimately affecting cellular responses to external stimuli. MLK3 shows widespread expression across different tissues and shares close relation to MLK1 and MLK2, highlighting MLK3′s significance in mitogen-activated protein kinase signaling pathways.

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MLK3 Antibody (D-11) References:

1. Growth factor signaling by receptor tyrosine kinases.  |  Schlessinger, J. and Ullrich, A. 1992. Neuron. 9: 383-91. PMID: 1326293
2. Signalling through SH2 and SH3 domains.  |  Mayer, BJ. and Baltimore, D. 1993. Trends Cell Biol. 3: 8-13. PMID: 14731533
3. MLK3 Signaling in Cancer Invasion.  |  Rattanasinchai, C. and Gallo, KA. 2016. Cancers (Basel). 8: PMID: 27213454
4. MLK3 phosphorylation by ERK1/2 is required for oxidative stress-induced invasion of colorectal cancer cells.  |  Schroyer, AL., et al. 2018. Oncogene. 37: 1031-1040. PMID: 29084209
5. The role of mixed lineage kinase 3 (MLK3) in cancers.  |  Ramachandraiah, K. and Thylur Puttalingaiah, R. 2022. Pharmacol Ther. 238: 108269. PMID: 36038020
6. TrkA expression directs the anti-neoplastic activity of MLK3 inhibitors in triple-negative breast cancer.  |  Nair, RS., et al. 2023. Oncogene. 42: 1132-1143. PMID: 36813855
7. MLK3 localizes mainly to the cytoplasm and promotes oxidative stress injury via a positive feedback loop.  |  Jiang, Y., et al. 2023. Cell Biochem Biophys. 81: 469-479. PMID: 37550525
8. MLK3 promotes prooncogenic signaling in hepatocellular carcinoma via TGFβ pathway.  |  Ke, R., et al. 2024. Oncogene. 43: 2307-2324. PMID: 38858590
9. Proteins with SH2 and SH3 domains couple receptor tyrosine kinases to intracellular signalling pathways.  |  Pawson, T., et al. 1993. Philos Trans R Soc Lond B Biol Sci. 340: 279-85. PMID: 8103930
10. PTK1, a novel protein kinase required for proliferation of human melanocytes.  |  Ezoe, K., et al. 1994. Oncogene. 9: 935-8. PMID: 8108137
11. MLK-3: identification of a widely-expressed protein kinase bearing an SH3 domain and a leucine zipper-basic region domain.  |  Ing, YL., et al. 1994. Oncogene. 9: 1745-50. PMID: 8183572
12. Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains.  |  Dorow, DS., et al. 1993. Eur J Biochem. 213: 701-10. PMID: 8477742

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MLK3 Antibody (D-11) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-MLK3 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MLK3 Antibody (D-11) Specifications

Alternate Product Name: MAP3K11

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 548-847

Epitope Target: MLK3

Epitope Species: human

Product Reactivity/Detection: MLK3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for MLK3 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin