MCT4 Antibody (D-1): sc-376140
- MCT4 Antibody (D-1) is a mouse monoclonal IgG2a κ MCT4 antibody, cited in 83 publications, provided at 200 µg/ml
- raised against amino acids 376-465 mapping within a C-terminal cytoplasmic domain of MCT4 of human origin
- MCT4 Antibody (D-1) is recommended for detection of MCT4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-MCT4 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for MCT4 Antibody (D-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with MCT4 Antibody (D-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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MCT4 Antibody (D-1) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Monocarboxylate Transporter 4 (MCT4) in mouse, rat, and human species through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-MCT4 antibody (D-1) specifically targets amino acids 376-465 within the C-terminal cytoplasmic domain of human MCT4, ensuring precise binding and reliable detection. MCT4 is a member of the solute carrier 16A (SLC16A) family of proteins, and is an integral membrane protein characterized by 10-12 transmembrane-helical domains, with both amino and carboxy termini located in the cytoplasm. This structural configuration facilitates proton-linked transport of lactate and other monocarboxylates across the plasma membrane. MCT4 (D-1) antibody plays a vital role in studying intracellular pH balance and sustained glycolytic activity, particularly in highly glycolytic tissues such as skeletal muscle and cancer cells where rapid lactate production is driven by the Warburg effect. Strategic localization of MCT4 on the plasma membrane effectively manages lactate levels, preventing intracellular acidification and supporting cellular metabolism and survival under anaerobic conditions. Understanding MCT4 structure underpins research into cancer metabolism and other diseases characterized by altered lactate transport. Anti-MCT4 antibody (D-1) is available in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile options for diverse experimental needs. MCT4 monoclonal antibody (D-1) supports research on expression, localization, and functional roles of MCT4 in various physiological and pathological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MCT4 Antibody (D-1) References:
- Lactate transport in skeletal muscle - role and regulation of the monocarboxylate transporter. | Juel, C. and Halestrap, AP. 1999. J Physiol. 517 (Pt 3): 633-42. PMID: 10358105
- The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation. | Halestrap, AP. and Price, NT. 1999. Biochem J. 343 Pt 2: 281-99. PMID: 10510291
- Lactate transporters (MCT proteins) in heart and skeletal muscles. | Bonen, A. 2000. Med Sci Sports Exerc. 32: 778-89. PMID: 10776897
- Abundance and subcellular distribution of MCT1 and MCT4 in heart and fast-twitch skeletal muscles. | Bonen, A., et al. 2000. Am J Physiol Endocrinol Metab. 278: E1067-77. PMID: 10827010
- Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats. | Gerhart, DZ., et al. 1997. Am J Physiol. 273: E207-13. PMID: 9252498
- Lactate transport in heart in relation to myocardial ischemia. | Halestrap, AP., et al. 1997. Am J Cardiol. 80: 17A-25A. PMID: 9293952
- Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past. | Price, NT., et al. 1998. Biochem J. 329 (Pt 2): 321-8. PMID: 9425115
- Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter. | Lin, RY., et al. 1998. J Biol Chem. 273: 28959-65. PMID: 9786900
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCT4 Antibody (D-1) | sc-376140 | 200 µg/ml | $322.00 | |||
MCT4 Antibody (D-1): m-IgGκ BP-HRP Bundle | sc-522851 | 200 µg Ab, 40 µg BP | $361.00 | |||
MCT4 Antibody (D-1) AC | sc-376140 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MCT4 Antibody (D-1) HRP | sc-376140 HRP | 200 µg/ml | $322.00 | |||
MCT4 Antibody (D-1) FITC | sc-376140 FITC | 200 µg/ml | $336.00 | |||
MCT4 Antibody (D-1) PE | sc-376140 PE | 200 µg/ml | $349.00 | |||
MCT4 Antibody (D-1) Alexa Fluor® 488 | sc-376140 AF488 | 200 µg/ml | $364.00 | |||
MCT4 Antibody (D-1) Alexa Fluor® 546 | sc-376140 AF546 | 200 µg/ml | $364.00 | |||
MCT4 Antibody (D-1) Alexa Fluor® 594 | sc-376140 AF594 | 200 µg/ml | $364.00 | |||
MCT4 Antibody (D-1) Alexa Fluor® 647 | sc-376140 AF647 | 200 µg/ml | $364.00 | |||
MCT4 Antibody (D-1) Alexa Fluor® 680 | sc-376140 AF680 | 200 µg/ml | $364.00 | |||
MCT4 Antibody (D-1) Alexa Fluor® 790 | sc-376140 AF790 | 200 µg/ml | $364.00 |