Anticorps Insulin (2D11-H5) recommandé pour la détection de Insulin d'origine mouse, rat et human par IP, IF, IHC(P) et ELISA

Anticorps Insulin (2D11-H5): sc-8033

Anticorps Insulin (2D11-H5) est évalué 4.9 de 5 de 22.
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Fiches techniques

    • Anti-L'anticorps Insulin 2D11-H5 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps Insulin fourni en 200 µg/ml
    • dirigé contre les acides aminés 1-84 représentant la protéine totale Insulin de porcine
    • Anti-L'Anticorps Insulin (2D11-H5) est recommandé pour la détection de Insulin d'origine mouse, rat et human par IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont et bovine and porcine
    • Anti-L'Anticorps Insulin (2D11-H5) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Insulin (2D11-H5): sc-8033.

Does this antibody capture proinsulin as well?

Posée par: Zoey111
Thank you for your question. Unfortunately, we do not have that information available for this insulin antibody.
Répondue par: Technical Support
Date de publication: 2020-07-09

How to dilute this antibody? What volumes can i use to arrive at 1:200?

Posée par: anjan1983
Thank you for your question. This antibody is provided as 200µg in 1ml (1000µl), which means you have 1µg of antibody per 5µl of solution. So to make a 1:200 dilution, you need to take 1µg (5µl of solution) into 1ml of your diluent (milk/blocking reagent).
Répondue par: Tech Service
Date de publication: 2019-08-30

what is the volume of primary antibody in the vial? sc-8033 200ug/ml?

Posée par: medha2001
Insulin Antibody (2D11-H5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg in a total volume of 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Répondue par: Tech Support Europe
Date de publication: 2019-08-30

Can this antibody be use to IF(frozen section)?

Posée par: Tienfen
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone).
Répondue par: Technical Support
Date de publication: 2019-04-22

Want to use antibody to distinguish proinsulin and mature insulin,what products are recommended?

Posée par: tinglan
Thank you for your question. Please contact our Technical Service Department and we can help recommend antibodies for the detection of proinsulin and mature insulin depending on what species and application(s) you need to use the antibodies in.
Répondue par: Technical Support
Date de publication: 2019-03-08

antibody: insulin antibody: prmary host : rabbit reactivity : mouse application : western blot conjugation: unconjugated Can i use this antibody for insulin secretion assay by western.

Posée par: firdos
Thank you for your question. Insulin Antibody (2D11-H5): sc-8033 is a mouse monoclonal antibody (raised in a mouse host). And it is not currently recommended for use in Western blot.
Répondue par: Technical Support
Date de publication: 2018-12-21

Can this antibody be used to detect canine insulin?

Posée par: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Répondue par: Technical Support 10
Date de publication: 2017-01-11

What is the recommended protocol for using this antibody in IHC(P)?

Posée par: AbPolly
Thank you for your question. For detailed protocol information, please contact our Technical Services Department or refer to our online protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support 10
Date de publication: 2017-01-11
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Rated 5 de 5 de par GREAT FEATURES. I purchased this antibody two weeks ago. It worked very well on pancreatic section. I will buy this antibody if I need it later. Shirley
Date de publication: 2019-06-27
Rated 5 de 5 de par Good Antibody for IHC Good signal on European seabass (Dicentrarchus labrax) fixed in Bouin's solution. Diluition 1:50
Date de publication: 2019-01-16
Rated 5 de 5 de par Immunohistochemistry: excellent results It works fine for Langerhans islets mouse and rat.
Date de publication: 2018-05-31
Rated 5 de 5 de par Works great! Works in hES cells at 1:50 dilution for immunofluorescence
Date de publication: 2018-03-01
Rated 5 de 5 de par Good anitibody for ELISA We got 10 ug sample and used for ELISA. It gave a good result, with 1:300 dilution, and can detect ng/ml level human insulin.
Date de publication: 2018-02-20
Rated 5 de 5 de par Easy to use on FFPE Mouse We received this as a sample. Before purchase a customer asked us to get a sample and work the antibody up. This is a straight forward, very easy, great looking antibody. Use a general protocol, sodium citrate pH6.0 dilution 1:100 - 1:250.
Date de publication: 2018-02-10
Rated 4 de 5 de par Very good got a free sample and used to perform western blot analysis on mouse hippocampi. The detected band is very clear, and seems specific.
Date de publication: 2018-02-08
Rated 5 de 5 de par Works beautifully Works beautifully to detect Insulin in murine tissues as confirmed Insulin KO mouse,1:600 dilution been used ,perfect result.
Date de publication: 2017-07-23
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